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United States Department of Agriculture

Agricultural Research Service

Research Project: CONTROL OF RUSTS OF CEREAL CROPS Title: Molecular cloning and characterization of two hypersensitive induced reaction genes from wheat infected by stripe rust pathogen

Authors
item Zhang, Gang - SHAANXI, CHINA
item Li, Yi-Min - SHAANXI, CHINA
item Wang, Xiao-Jie - SHAANXI, CHINA
item Xia, Ning - SHAANXI, CHINA
item Dong, Yan-Ling - SHAANXI, CHINA
item Zhang, Yi - SHAANXI, CHINA
item Guo, Jun - SHAANXI, CHINA
item Wei, Guo-Rong - SHAANXI, CHINA
item Huang, Li-Li - SHAANXI, CHINA
item Chen, Xianming
item Kang, Zhen-Sheng - SHAANXI, CHINA

Submitted to: Biologia Plantarum
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 3, 2012
Publication Date: December 20, 2012
Citation: Zhang, G., Li, Y., Wang, X., Xia, N., Dong, Y., Zhang, Y., Guo, J., Wei, G., Huang, L., Chen, X., Kang, Z. 2012. Molecular cloning and characterization of two hypersensitive induced reaction genes from wheat infected by stripe rust pathogen. Biologia Plantarum. 55(4): 696-702.

Interpretive Summary: Identification of wheat genes induced by stripe rust infection is important for understanding the molecular mechasnisms of the plant-pathogen interactions, but not much information is available for these genes. In this study, we identified a novel gene, TaHIR2, that was induced a wheat genotype producing a hypersensitive reaction (HIR) using in silico cloning. TaHIR2 was deduced to encode a 284-amino acid protein, whose molecular mass and isoelectric point were 31.05 kD and 5.18, respectively. Amino acid sequence analysis identified some interetsing structures . Phylogenetic analysis of 13 HIR genes from different monocots indicated that TaHIR2 was highly homologous to HvHIR2 in barley. Transient expression analysis using particle-mediated bombardment showed that the TaHIR2 fusion protein was located in the onion epidermal cells. Quantitative RT-PCR analyses revealed that TaHIR2 transcripts were significantly accumulated in adult wheat leaves with maximum induction at 18 hours post inoculation with stripe rust, whereas slightly up-regulation could also be observed in the compatible reaction at the seedling stage. These results suggest that TaHIR2 may play an active role in wheat defense against stripe rust.

Technical Abstract: A novel gene induced during hypersensitive reaction (HIR) in wheat was identified using in silico cloning and designated as TaHIR2. The TaHIR2 gene was deduced to encode a 284-amino acid protein, whose molecular mass and isoelectric point (pI) were 31.05 kD and 5.18, respectively. Amino acid sequence analysis demonstrated the presence of stomatins, prohibitin, flotillins, HflK/C (SPFH) domain and prohibitin homologue for the TaHIR2 protein. Phylogenetic analysis of 13 HIR genes from different monocots indicated that TaHIR2 was highly homologous to HvHIR2. Transient expression analysis using particle-mediated bombardment showed that the TaHIR2 fusion protein was located in the onion epidermal cells. Quantitative RT-PCR analyses revealed that TaHIR2 transcripts were significantly accumulated in adult wheat leaves with maximum induction at 18 h post inoculation with the stripe rust, whereas slightly up-regulation could also be observed in the compatible reaction at the seedling stage. These results suggest that TaHIR2 may play an active role in wheat defense against stripe rust.

Last Modified: 10/25/2014
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