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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR ANALYSIS OF VIRULENCE DETERMINANTS OF SELECT BACTERIA IN FISH DISEASES Title: Channel catfish (Ictalurus punctatus) CD156a mRNA sequence

Authors
item Yeh, Hung-Yueh
item Klesius, Phillip

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: February 9, 2009
Publication Date: August 30, 2009
Citation: Yeh, H., Klesius, P.H. 2009. Channel catfish (Ictalurus punctatus) CD156a mRNA sequence. Genbank. Accession No. FJ594762.

Technical Abstract: CD156a, also known as A Disintegrin And Metalloproteinase domain 8 (ADAM8), is a member of ADAM family. We cloned, and sequenced the channel catfish CD156a transcript. The full-length of the CD156a cDNA consisted of 3035 nucleotides, including a 5’-untranslated region (UTR), an open reading frame and a 3’-UTR. In the 5’-UTR, the sequence had a Kozak sequence (A/GNNATG) (Kozak, 1989). The 3’-UTR had 451 nucleotides in length, and contained three canonical features of mRNA: (1) an mRNA instability motif (attta), (2) a polyadenylation signal sequence (aataa) and (3) a 28-nucleotide polyadenylation tail. The open reading frame of the channel catfish CD156a transcript appears to encode a 850-amino acid residue peptide with a calculated molecular mass of 94.58 kDa and pI of 7.96 at pH 7.0. The peptide had three potential N-glycosylation sites-Asn82, Asn115 and Asn170 (numbering after the channel catfish CD156a peptide).

Last Modified: 10/20/2014
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