FUNCTIONAL GENOMICS AND GENETIC ANALYSIS OF THE INNATE IMMUNE RESPONSE REQUIRED TO RESIST FOOD-BORNE BACTERIAL INFECTIONS IN POULTRY
Location: Food and Feed Safety Research
Title: Differential AP-1 and NF-kB expression in polymorphonuclear cells from a non-mammalian species following TLR4-mediated stimulation
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: January 15, 2009
Publication Date: March 29, 2009
Citation: Swaggerty, C.L., Street, M.R., Kogut, M.H. 2009. Differential AP-1 and NF-kB expression in polymorphonuclear cells from a non-mammalian species following TLR4-mediated stimulation [abstract]. Keystone Symposia: Pattern Recognition Molecules and Immune Senors of Pathogens, March 29-April 3, 2009, Banff, Alberta. p. 127.
Previously, evaluation of heterophil function, susceptibility to pathogenic challenge, and pro-inflammatory cytokine mRNA expression levels showed differences between two parental lines of chickens. Line A is always more responsive and more resistant compared to Line B. Based on these findings, we hypothesized the differential response was due to, in part, differences in protein expression of the transcription factors within the AP-1 and NF-kappabeta families. Heterophils, the avian equivalent to neutrophils, were isolated from 4-day-old chickens, stimulated with live Salmonella enteritidis or purified lipopolysaccharide, and protein expression levels of the AP-1 (c-jun, c-fos, FosB, Fra-1, JunD, and JunB) and NF-kappabeta (p50, p52, p65, c-Rel, and Rel-B) families quantitated. Following SE-treatment, c-jun and FosB levels were higher (p less than or equal to 0.004) in line A while line B was unchanged. Expression of p52 was also higher (p less than or equal to 0.01) in line A heterophils compared to line B after SE-treatment. There was a slight down-regulation in line B following treatment with SE. These data indicate increased responsiveness of Line A chickens and/or their heterophils is influenced, in part, by a differential transcription factor profile including members of the AP-1 and NF-kappabeta families.