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United States Department of Agriculture

Agricultural Research Service

Research Project: SOYBEAN DISEASE AND PEST MANAGEMENT Title: Global Gene Expression Profiles of Resistant and Susceptible Genotypes of Glycine tomentella During Phakopsora pachyrhizi Infection

Authors
item Soria-Guerra, Ruth Elena -
item Rosales-Mendoza, Sergio -
item Chang, Sungyul -
item Haudenshield, James -
item Padmanaban, Annamalai -
item Rodriguez-Zas, Sandra -
item Hartman, Glen
item Ghabrial, Said A -
item Korban, Schuyler -

Submitted to: The Plant Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 11, 2009
Publication Date: April 1, 2010
Citation: Soria-Guerra, R., Rosales-Mendoza, S., Chang, S., Haudenshield, J.S., Padmanaban, A., Rodriguez-Zas, S., Hartman, G.L., Ghabrial, S., Korban, S.S. 2010. Global Gene Expression Profiles of Resistant and Susceptible Genotypes of Glycine tomentella During Phakopsora pachyrhizi Infection. The Plant Genome. 120:1315-1333.

Interpretive Summary: Soybean rust, caused by Phakopsora pachyrhizi, is a destructive foliar disease of soybean. Towards the goal of identifying genes that control resistance to soybean rust, a soybean rust resistant Glycine tomentella genotype (PI509501) and a soybean rust susceptible G. tomentella genotype (PI441011) were inoculated with the rust fungus. Among 38,000 genes monitored by microarray analysis, 1342 genes showed statistically significant differential expression between uninfected and P. pachyrhizi-infected leaves. Differentially expressed genes were grouped into 12 functional categories. These results provide insights into mechanisms underlying resistance and general activation of plant defense pathways in response to rust infection. This information is important to soybean pathologists, plant molecular genetists and others that are interested in basic studies on host-pathogen interactions.

Technical Abstract: Soybean rust, caused by Phakopsora pachyrhizi, is a destructive foliar disease that occurs in many soybean-producing countries. Towards the goal of identifying genes controlling resistance to soybean rust, transcriptome profiling was conducted in resistant and susceptible Glycine tomentella genotypes triggered by P. pachyrhizi-infection. Among 38,000 genes monitored by microarray analysis, at 5% false discovery rate, 1342 genes were identified that showed statistically significant differential expression between uninfected and P. pachyrhizi-infected leaves at 12, 24, 48 and 72 h post-inoculation, in both rust-susceptible and -resistant genotypes. Differentially expressed genes were grouped into 12 functional categories, and among those, large numbers relate to basic plant metabolism. Transcripts for genes involved in the phenylpropanoid pathway were up-regulated early during rust infection. Similarly, genes coding for proteins related to stress and defense responses such as glutathione-S-transferases, peroxidases, heat shock proteins, and lipoxygenases were consistently up-regulated following infection at all four time points. Whereas, subsets of genes involved in cellular transport, cellular communication, cell cycle, and DNA processing were down-regulated. Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) confirmed results of the microarray analysis. These findings provided insights into mechanisms underlying resistance and general activation of plant defense pathways in response to rust infection.

Last Modified: 11/24/2014
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