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United States Department of Agriculture

Agricultural Research Service

Research Project: IDENTIFICATION AND CHARACTERIZATION OF PEST INSECT IMMUNE RESPONSES TO BIOLOGICAL CONTROL AGENTS Title: Heliothine Immunogenomics

Author
item Shelby, Kent

Submitted to: Entomological Society of America Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 2009
Publication Date: December 15, 2009
Citation: Shelby, K. 2009. Heliothine Immunogenomics [abstract]. Entomological Society of America Annual Meeting. p. 103.

Technical Abstract: Resistance of Heliothine pests to microbial entomopathogens and to parasitoids is one barrier to effective control by biological control agents. In order to better understand the immune response of budworms and bollworms against microbial entomopathogens, several orthologs of antibacterial response proteins were identified and extracted from a budworm EST database for detailed study of functional genomics. Induction of these selected antibacterial protein transcripts by fungal, bacterial and baculoviral infection was quantitated using qPCR. Transcript levels of immediate innate defense such as heliomicin, virescein, viresin, gloverin, lebocin, gloverin, ABP-2, cecropins, attacins and others were rapidly elevated by bacterial elicitation, usually as early as three hours post-treatment. In contrast, the transcript levels of other immune responders such as scolexin-B, growth-blocking peptide binding protein, C-type lectin and serpin-3 were more slowly induced by bacterial elicitation.

Last Modified: 10/26/2014
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