|Ozek, Gulmira -|
|Demirci, Fatih -|
|Ozek, Temel -|
|Khan, Shabana -|
|Baser, K. Husnu Can -|
|Duran, Ahmet -|
|Hamzaoglu, Ergin -|
Submitted to: Journal of Chromatography A
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 27, 2009
Publication Date: January 12, 2010
Citation: Ozek, G., Demirci, F., Ozek, T., Tabanca, N., Wedge, D.E., Khan, S.I., Baser, K., Duran, A., Hamzaoglu, E. 2010. Gas Chromatographic-Mass Spectrometric Analysis of Volatiles Obtained by Four Different Techniques from Salvia rosifolia Sm. and Evaluation for Biological Activity. Journal of Chromatography A. 1217(2010):741-748. Interpretive Summary: Microwave heating has already been widely applied in solvent extraction because of its main advantages like rapidity and high efficiency. Many cases have already proved that microwave assisted extraction is a viable alternative to conventional techniques for many kinds of samples. Volatile constituents from the aerial parts of Salvia rosifolia Sm. (Lamiaceae), endemic to Turkey, were obtained by four different isolation techniques and then analyzed by gas chromatography (GC/FID) and gas chromatography – mass spectrometry (GC/MS) methods. By using GC–MS system the compositions of essential oil extracted by applying four kinds of extraction methods were identified. There was no obvious difference in the quality of essential oils obtained by the four kinds of extraction methods. Salvia oil was also evaluated against human and plant pathogens.
Technical Abstract: Volatile constituents from the aerial parts of Salvia rosifolia Sm. (Lamiaceae), endemic to Turkey, were obtained by four different isolation techniques and then analyzed by gas chromatography (GC/FID) and gas chromatography – mass spectrometry (GC/MS) methods. Also in scope of the present work, the hydrodistilled oil of S. rosifolia was evaluated for antibacterial, antifungal, anticancer, antioxidant and cytotoxic activities. Conventional hydrodistillation (HD), microwave assisted hydrodistillation (MWHD), microdistillation (MD) and microsteam distillation – solid phase microextraction (MSD-SPME) techniques performed for 180 min, 45 min, 55 min and 0.5 min, respectively, yielded volatiles of similar profile. HD and MWHD techniques produced quantitatively (yield, 0.39% and 0.40%) and qualitatively (aromatic profile) similar essential oils. a-Pinene, 1,8-cineole, B-pinene, B-caryophyllene and caryophyllene oxide were identified as major constituents of S. rosifolia volatiles. In vitro broth micro-dilution methods were used to evaluate activity against human pathogenic Gram (+) and Gram (-) bacterial strains and Candida albicans. Salvia rosifolia oil was found effective against Methicillin-resistant Staphylococcus aureus (MRSA) with a MIC value of 125 µg/mL. Other human pathogenic microorganisms were also inhibited within a moderate range (MIC = 125-1000 µg/mL). Antifungal activity of the oil was also observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides using the direct overlay bioautography assay. No cytotoxicity was observed for S. rosifolia oil up to 25 µg/mL against malignant melanoma, epidermal, ductal and ovary carcinoma.