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United States Department of Agriculture

Agricultural Research Service

Research Project: CONTROL OF FUSARIUM VERTICILLIOIDES, FUMONISINS AND FUSARIUM DISEASES OF MAIZE

Location: Bacterial Foodborne Pathogens & Mycology Research Unit

Title: Comparative Genomics Reveals Mobile Pathogenicity Chromosomes in Fusarium

Authors
item Ma, Li-Jun -
item Borkovich, Katherine -
item Coleman, Jeffrey -
item Daboussi, Marie-Josee -
item Pietro, Antonio -
item Dufresne, Marie -
item Freitag, Michael -
item Grabherr, Manfred -
item Henrissat, Bernard -
item Kang, Seogchan -
item Park, Jongsun -
item Rep, Martijn -
item Shim, Won-Bo -
item Woloshuk, Charles -
item Xie, Xiaohui -
item Xu, Jin-Rong -
item Antoniw, John -
item Baker, Scott -
item Bluhm, Burton -
item Breakspear, Andrew -
item Brown, Daren
item Butchko, Robert
item Chapman, Sinead -
item Coulson, Richard -
item Coutinho, Pedro -
item Danchin, Etienne G.J. -
item Diener, Andrew -
item Gale, Liane -
item Gardiner, Donald -
item Goff, Stephen -
item Hammond-Kosack, Kim -
item Hilburn, Karen -
item Hua-Van, Aurelie -
item Jonkers, Wilfried -
item Kazan, Kemal -
item Kodira, Chinnappa -
item Koehrsen, Michael -
item Kumar, Lokesh -
item Lee, Yong-Hwan -
item Li, Liande -
item Manners, John -
item Miranda-Saavedra, Diego -
item Mukherjee, Mala -
item Park, Gyungsoon -
item Park, Jongsun -
item Park, Sook-Young -
item Proctor, Robert
item Regev, Aviv -
item Ruiz-Roldan, M. Carmen -
item Sain, Divya -
item Sakthikumar, Sharadha -
item Sykes, Sean -
item Schwartz, David -
item Turgeon, Gillian -
item Wapinski, Ilan -
item Yoder, Olen -
item Young, Sarah -
item Zeng, Qiandong -
item Zhou, Siguo -
item Galagan, James -
item Cuomo, Christina -
item Kistler, H

Submitted to: Nature
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 20, 2010
Publication Date: March 18, 2010
Citation: Ma, L., Borkovich, K.A., Coleman, J.J., Daboussi, M., Pietro, A.D., Dufresne, M., Freitag, M., Grabherr, M., Henrissat, B., Kang, S., Park, J., Rep, M., Shim, W., Woloshuk, C., Xie, X., Xu, J., Antoniw, J., Baker, S.E., Bluhm, B.H., Breakspear, A., Brown, D.W., Butchko, R.A., Chapman, S., Coulson, R., Coutinho, P.M., Danchin, E., Diener, A., Gale, L., Gardiner, D., Goff, S., Hammond-Kosack, K.E., Hilburn, K., Hua-Van, A., Jonkers, W., Kazan, K., Kodira, C.D., Koehrsen, M., Kumar, L., Lee, Y., Li, L., Manners, J., Miranda-Saavedra, D., Mukherjee, M., Park, G., Park, J., Park, S., Proctor, R., Regev, A., Ruiz-Roldan, M., Sain, D., Sakthikumar, S., Sykes, S., Schwartz, D.C., Turgeon, G., Wapinski, I., Yoder, O., Young, S., Zeng, Q., Zhou, S., Galagan, J., Cuomo, C.A., Kistler, H.C. 2010. Comparative Genomics Reveals Mobile Pathogenicity Chromosomes in Fusarium. Nature. 464(7287):367-373.

Interpretive Summary: The results of this research demonstrate differences in genome sequences that affect toxin production and ability to cause plant disease in the fungus Fusarium. Species of this fungus are among the most economically important fungal pathogens of crop plants because they occur worldwide and on many crops, they can reduce crop yield and quality, and they can produce toxins (mycotoxins) that are hazards to human and animal health. Here, the whole genome sequence was determined for two Fusarium species, one that causes disease on tomato and a second that causes disease on corn. The sequences were compared with a previously determined whole genome sequence of a third species that causes disease on wheat, corn, and barley. The results demonstrate that the genome sequences are highly similar in the three species, but that important differences exist. Some of the genes that differ among the three species are required for production of different toxic metabolites, whereas others are important for ability to cause disease on crop hosts. These findings provide information that plant breeders and plant biotechnologists can use to improve the resistance of crops to diseases caused by Fusarium and to reduce mycotoxin contamination of crops.

Technical Abstract: Fusarium species are among the most important phytopathogenic and toxigenic fungi, having significant impact on crop production and animal health. Distinctively, strains of F. oxysporum exhibit wide host range and are pathogenic to both plant and animal species, reflecting remarkable genetic adaptability. To understand the mechanism underlying such genetic plasticity and rapid pathogenic development, we compared the genomes of three economically important and phylogenetically related, yet phenotypically distinct phytopathogenic species, F. graminearum, F. verticillioides and F. oxysporum f. sp. lycopersici. Comparative analysis revealed diverse and actively transcribed secondary metabolite biosynthetic clusters in F. graminearum and F. verticillioides as well as greatly expanded lineage-specific (LS) genomic regions in F. oxysporum that include four entire chromosomes and account for more than one-quarter of the genome. LS regions are rich in transposons and genes involved in host-pathogen interactions, including known effectors, enzymes targeting plant substrates or processes, and genes involved in lipid signalling and gene silencing. We found evidence for the acquisition of the LS chromosomes through horizontal transfer, which may explain the polyphyletic origin of host specificity in F. oxysporum and the rapid emergence of new pathogenic lineages in distinct genetic backgrounds.

Last Modified: 9/22/2014
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