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United States Department of Agriculture

Agricultural Research Service

Research Project: GENOMICS AND MOLECULAR APPROACHES TO WHEAT TRAITS

Location: Genomics and Gene Discovery

Title: Radiation hybrid mapping in crop plants

Authors
item Kalavachrla, Venu -
item Hossia, Khwaja -
item GU, YONG
item Maan, Shivcharan -
item Kianian, Shahryar -

Submitted to: Advances in Agronomy
Publication Type: Review Article
Publication Acceptance Date: December 15, 2008
Publication Date: May 15, 2009
Citation: Kalavachrla, V., Hossia, K., Gu, Y.Q., Maan, S., Kianian, S. 2009. Radiation hybrid mapping in crop plants. Advances in Agronomy. 102:199-219.

Interpretive Summary: Crop mapping determines the linear order of genes along a chromosome. Various mapping technologies are currently available. In this review paper, we introduce radiation hybrid mapping in crop plants. Radiation hybrid mapping technology provides a useful tool to generate a high resolution physical map for complex crop genomes including wheat. The resource generated by this technology can be directly used for mapping and cloning important genes for crop improvement.

Technical Abstract: Map-based cloning and manipulation of genes controlling important traits for crop remains a great challenge due to the complex of crop genomes and lack of a high resolution of genetic and physical maps. In this review article, we compared the various mapping methods available for plant research and introduce radiation hybrid mapping technology potential useful for generating high resolution physical maps for complex genomes such as wheat. Unlike genetic mapping, radiation hybrid mapping is not recombination- based; making marker distribution more evenly along a chromosome. Therefore, the use of a combination of genetic and physical mapping methods can be very useful for advancing knowledge on the number and distance between genes of interests as well as to better understand the topography and organization of the genome.

Last Modified: 9/10/2014
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