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United States Department of Agriculture

Agricultural Research Service

Research Project: INTEGRATION OF NUTRITIONAL, GENETIC AND PHYSIOLOGICAL APPROACHES TO IMPROVE PRODUCTION EFFICIENCY OF RAINBOW TROUT Title: Development of PCR-Based DNA markers flanking three low phytic acid mutant loci in barley

Authors
item Oliver, Rebekah
item Yang, Chunyan -
item Hu, Gongshe
item Raboy, Victor
item Zhang, Mengchen -

Submitted to: Molecular Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 10, 2009
Publication Date: July 10, 2009
Repository URL: http://riley.nal.usda.gov/nal_web/digi/submission.html
Citation: Oliver, R.E., Yang, C., Hu, G., Raboy, V., and Zhang, M. 2009. Identification of PCR- based DNA markers flanking three low phytic acid mutant loci in barley. Journal of plant breeding and crop science 1: 87-93

Interpretive Summary: Phytic acid (PA) is the most abundant form of phosphorus (P) in cereal grains. PA chelates mineral cations to form an indigestible salt, and is thus regarded as an antinutritional agent and a contributor to water pollution. Use of lpa lines in breeding has proved a practical approach for improvement of phosphorus nutrition in barley. Development of closely linked DNA markers, particularly PCR-based markers, will greatly enhance the marker-assistant selection for the low phytic acid trait in the breeding process. Due to the multiple lpa loci have been identified in barley, combinations of different loci could be alternative approach to better improve the phosphorus contents without compromising the important agronomic traits. To better track each lpa gene or allele in combination of multiple lpa loci requires flanking DNA markers for each lpa locus. In this report , we developed PCR-based markers flanking three major lpa loci in barley: lpa1-1 (M422), lpa2-1 linked locus (M640), and a locus linked to the myo-inositol 1-phosphate synthase (MIPS) gene (M678). In addition, marker position accuracy in the MIPS region has been improved by detection and elimination of marker redundancy.

Technical Abstract: Phytic acid (PA) is the most abundant form of phosphorus (P) in cereal grains. PA chelates mineral cations to form an indigestible salt, and is thus regarded as an antinutritional agent and a contributor to water pollution. Grain with low phytic acid (lpa) genotypes could aid in mitigating this problem. In barley, more than 20 lpa mutant lines have been isolated, representing at least six different genetic loci. These mutants have significantly reduced levels of seed PA, which are largely matched for by inorganic P, a form readily digestable by animals. Use of lpa lines in breeding has proved a practical approach for improvement of phosphorus nutrition in barley. Efficient utilization of these loci in marker-assisted selection breeding programs requires identification of closely-linked, high-throughput molecular markers. Here we report development of flanking, PCR-based markers for three major lpa loci in barley: lpa1-1 (M422), lpa2-1 linked locus (M640), and a locus linked to the myo-inositol 1-phosphate synthase (MIPS) gene (M678). In addition, marker position accuracy in the MIPS region has been improved by detection and elimination of marker redundancy.

Last Modified: 11/28/2014
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