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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVED KNOWLEDGE OF VIRULENCE FACTORS TO DEVELOP POSTHARVEST DECAY CONTROL STRATEGIES Title: Temperature suppresses decay on apple fruit by affecting Penicillium solitum conidial germination, mycelial growth and polygalacturonase activity

Authors
item Vico, Ivana -
item Jurick, Wayne
item Camp, Mary
item Janisiewicz, Wojciech
item Conway, William

Submitted to: Plant Pathology Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 22, 2010
Publication Date: September 2, 2010
Repository URL: http://hdl.handle.net/10113/31693
Citation: Vico, I., Jurick Ii, W.M., Camp, M.J., Janisiewicz, W.J., Conway, W.S. 2010. Temperature suppresses decay on apple fruit by affecting Penicillium solitum conidial germination, mycelial growth and polygalacturonase activity. Plant Pathology Journal. 9(3):144-148.

Interpretive Summary: Blue mold caused by fungal plant pathogens, Penicillium expansum and P. solitum, cause major economic losses on apple fruit in storage. During this study, the effects of various temperatures commonly used by packers and grocery stores were implemented to determine if they were able to deter growth of both blue mold fungi. We found that low temperatures slowed or inhibited pathogen growth on apple fruit when kept at low temperature. We also determined that other aspects of fungal growth in culture (i.e. sporulation, mycelia growth, conidial germination etc.) were also affected by cold storage temperatures. We also examined the effect of temperature on an enzyme involved in virulence which was temperature responsive. Data from this study is useful and can directly benefit apple growers, packers, grocery stores, and consumers.

Technical Abstract: Penicillium solitum causes blue mold on apples during storage which results in economic losses. Information pertaining to growth and decay caused by this pathogen is important for developing disease control strategies. Therefore, we evaluated the effect of temperature on decay caused by P. solitum in apples, fungal growth in culture, and quantitatively and qualitatively assayed polygalacturonase (PG) activity. Decay was evident only on apple fruit incubated at 20°C following 21 days incubation. However, decay developed when apples were moved from low temperatures (0, 5 and 10) to 20°C, which shows that the inoculum was viable following cold exposure. P. solitum viability at low temperature was confirmed as decay developed on inoculated apples following incubation for 3 months at 0°C. Lower temperatures reduced conidial germination and mycelial growth on PDA, which may explain the delay in decay development in inoculated fruit which was stored at 0, 5, and 10°C. Crude extracts from P. solitum decayed apple fruit tissue possessed detectable PG activity using the qualitative plate assay at 20°C but not at 0, 5, or 10°C. However, a quantitative method (Gross, 1982) for determining in vitro PG activity using purified enzyme was observed at 0, 5, 10 and 20°C and decreased with temperature. Although P. solitum is able to decay apple fruit following prolonged cold storage, low temperature is beneficial as it delays lesion development by affecting various factors of pathogen virulence.

Last Modified: 10/23/2014
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