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United States Department of Agriculture

Agricultural Research Service

Research Project: BIOCONTROL OF FUMONISIN AND OTHER MYCOTOXINS IN CORN AND TALL FESCUE WITH MICROBIAL ENDOPHYTES Title: A new species of Jahnulales from Las Ilusiones Lagoon, Tabasco, Mexico

Authors
item Gonzalez, Maria -
item Glenn, Anthony
item Rosique-Gil, Edmundo -
item Hanlin, Richard -
item Hyde, Kevin -

Submitted to: Mycological Symposium Asian Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 15, 2009
Publication Date: November 15, 2009
Citation: Gonzalez, M.C., Glenn, A.E., Rosique-Gil, E., Hanlin, R.T., Hyde, K.D. 2009. A new species of Jahnulales from Las Ilusiones Lagoon, Tabasco, Mexico. Mycological Symposium Asian Proceedings. November 15 - 19, 2009. Taichung, Taiwan.

Interpretive Summary: Abstract - no summary required.

Technical Abstract: During a study on biodiversity of freshwater ascomycetes from an urban tropical lagoon, an ascomycete with similar morphology to species of Jahnulales was obtained. Smooth surface test blocks of Pinus sp., Bucida sp., Cedrela sp. and Tabebuia sp. were submerged in pairs close to a private house wharf during 12 weeks in the Las Ilusiones Lagoon, located in the central section of Villahermosa City, in the State of Tabasco, Mexico. The test blocks were pulled out of the water and placed in separate sterile Ziploc® plastic bags, transported at ambient temperatures to Mexico City laboratory and processed within 6h. During the removal of the test blocks, water samples were collected and temperature, pH, dissolved oxygen, organic matter and phosphate were measured. Collected material was washed in tap water and placed in moist chambers assembled with transparent plastic boxes with hermetic covers and a layer of moistened paper towels in the bottom of the box. Samples were incubated at laboratory conditions (25-30 C) under natural daylight. Wood panels were examined at 4 weeks for the presence of ascomata. Squash mounts of ascomata were prepared on glass slides in sterile water and ascomata, asci and ascospores were measured and photographed using light/interference/phase contrast microscopy. The fungus was preserved dried on wood panels and mounted in glycerin in permanent microscope slides prepared according to the double cover glass method. Axenic isolates of this ascomycete were made by picking up ascospores from the spore mass found at the tip of the ascoma and transfered to the surface of agar media. The dried specimens and cultures were deposited in the Coleccion de Hongos del Herbario Nacional (MEXU). For molecular procedures the internal transcribed spacer (ITS1-5.8S rDNA-ITS2) region of nuclear ribosomal DNA from the new ascomycete was amplified and sequenced. The Mexican ascomycete is characterized by its ascomata on wood, gregarious or individual, superficial to partly immersed globose to subglobose, sessile, coriaceous, dark brown, ostiolate, with short to long neck, light brown, straight or convoluted, sometimes bifurcated, hyaline and ornamented with short hairs when young. Pseudoparaphyses septate, hyaline, branched. Asci clavate, bitunicate, fissitunicate, persistent, 8-spored. Ascospores fusiform, 2-celled, constricted at the septum, hyaline, smooth walled, pale brown, multiguttulate, with a gelatinous sheath, not appendaged. Colonies on V8 vegetable juice agar (V8A) produced dark immersed mycelium formed by wide diameter hyphae. Based on morphological and molecular analyses, the freshwater Mexican ascomycete belongs in the Jahnulales and is close but separate from the species of Aliquandostipite clade.

Last Modified: 10/30/2014
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