Technical Abstract: The gboma (S. macrocarpon L.) and scarlet eggplants (S. aethiopicum L.) can be crossed with the common eggplant and represent a genetic resource of interest for improving several traits of common eggplant. This work is focused on the analysis of glycoalkaloid (GA) content on S. macrocarpon and S. aethiopicum eggplants. Liquid Chromatography-Mass Spectrometry with selective-ion monitoring (SIM) was used to quantify GAs in S. macrocarpon and S. aethiopicum compared to common eggplant (S. melongena L.). Fruits of S. aethiopicum and S. melongena presented 0.58 – 4.56 mg/100 g of '-solamargine (SM) and 0.17 – 1 mg/100 g of '-solasonine (SS), on wet basis. On the other hand, S. macrocarpon fruits had much higher values, with 124 – 197 mg/100 g of SM and 16 – 23 mg/100 g of SS. However, the proportions of SM and SS were similar between fruits of S. melongena and S. macrocarpon (76 – 89 % of SM), while in S. aethiopicum fruit composition was more variable (48 – 89 % of SM). According to these results, S. macrocarpon fruits, with values of GAs 5 to 10 times higher than the value considered safe in foods, should not be considered suitable for human consumption; however, the analysed fruits of S. aethiopicum were similar to those of S. melongena (about 1/7 of values considered as toxic), and could be considered safe for consumption. Also, because of its very low levels of toxic GAs, they represent an alternative to wild species for being incorporated into eggplant breeding programmes in order to obtain new improved varieties of common eggplant.