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United States Department of Agriculture

Agricultural Research Service

Research Project: EVOLUTIONARY ENZYMES AND SEPARATION PROCESSES FOR IMPROVED BIOREFINING OF CROPS AND RESIDUES Title: Development and validation of a hydrophilic interaction liquid chromatographic method for determination of aromatic amines in environmental water

Authors
item Li, Ruiping -
item Zhang, Yi -
item Lee, Charles
item Lu, Rongrong -
item Huang, Yingping -

Submitted to: Journal of Chromatography A
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 8, 2010
Publication Date: October 13, 2009
Citation: Li, R., Zhang, Y., Lee, C.C., Lu, R., Huang, Y. Development and validation of a hydrophilic interaction liquid chromatographic method for determination of aromatic amines in environmental water. Journal of Chromatography, 2010. A 1217(11), pp 1799-1805.

Interpretive Summary: Toxic aromatic amines, such as aniline and other substituted derivatives, are important industrial chemicals that are used to make dyes, synthetic polymers, rubbers, pesticides, cosmetics, medicines, and many other chemicals. Given the increasing use of these compounds in various industries, monitoring of their levels in environmental waters is of critical importance to the protection of human health and the environment. This report describes the development of a simple, precise, and accurate chromatographic method for the determination of 5 aromatic amines in environmental water samples.

Technical Abstract: A simple, precise, and accurate hydrophilic interaction liquid chromatographic (HILIC) method has been developed for the determination of 5 aromatic amines in environmental water samples. Chromatography was carried out on a bare silica column, using a mixture of acetonitrile: phosphate buffer (10 mM, pH 1.5) (85:15, v/v) as a mobile phase at a flow rate of 1 mL•min-1. Aromatic amines were detected by UV absorbance at 254 nm. The linear range of amines was good (r>0.999) and limit of detection (LOD) within 0.05-0.2 mg•L-1 (S/N=3). The retention mechanism for the analytes under optimum conditions was determined to be a combination of adsorption, partition and ionic interactions, with adsorption being the most influential parameter. The proposed method was applied to environmental water samples. Aromatic amines were isolated from aqueous samples using solid-phase extraction (SPE) with Oasis HLB cartridges. Recoveries of greater than 76% with precision (RSD) less than 10.7% were obtained at amine concentrations of 2.5-50 µg•L-1 from 100 mL river water. The present HILIC technique proved to be a viable method for the analysis of aromatic amines in environmental water samples.

Last Modified: 11/28/2014
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