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United States Department of Agriculture

Agricultural Research Service

Research Project: Germplasm Enhancement and Genetic Improvement of Cotton

Location: Genetics and Precision Agriculture Research

Title: Cotton Chromosome Substitution Lines Crossed with Cultivars: Genetic Model Evaluation and Seed Trait Analyses

Authors
item Wu, Jixiang -
item McCarty, Jack
item Jenkins, Johnie

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 23, 2009
Publication Date: February 1, 2010
Citation: Wu, J., McCarty Jr., J.C., Jenkins, J.N. 2010. Cotton Chromosome Substitution Lines Crossed with Cultivars: Genetic Model Evaluation and Seed Trait Analyses. Theoretical and Applied Genetics. 120:1473-1483.

Interpretive Summary: Cottonseed provides a desirable and important nutrition profile. Seed traits for 13 cotton chromosome substitution lines, five cultivars and their F3 hybrids were evaluated. The seed traits were protein content, oil content, seed hull fiber content, seed index, seed volume, and embryo percentage. Oil and protein were expressed as a percentage of seed weight. An index was calculated for seed traits which is the grams of product per 100 seed. An additive and dominance genetic model with cytoplasmic effects was designed, assessed, and used to analyze the seed traits. Cytoplasmic effects, additive effects and dominance effects were detected for oil index and several other seed traits. Cytoplasmic and additive effects for parents and dominance effects in both homozygous and heterozygous forms were also predicted. Chromosome associations with additive and dominance effects were also detected in this study. Favorable genetic effects were predicted in this study and the results provided evidence that these seed traits can be genetically improved.

Technical Abstract: Seed from Upland cotton, Gossypium hirsutum L., provides a desirable and important nutrition profile. In this study, six seed traits (protein content, oil content, seed hull fiber content, seed index, seed volume, embryo percentage) for F3 hybrids of 13 cotton chromosome substitution lines crossed with five elite cultivars over four environments were evaluated. Oil and protein were expressed both as percentage of total seed weight and as an index which is the grams of product/100 seed. An additive and dominance (AD) genetic model with cytoplasmic effects was designed, assessed by simulations, and employed to analyze these seed traits. Simulated results showed that this model was sufficient for analyzing the data structure with F3 and parents in multiple environments without replications. Significant cytoplasmic effects were detected for seed oil content, oil index, seed index, seed volume, and seed embryo percentage. Additive effects were significant for protein content, fiber content, protein index, oil index, fiber index, seed index, seed volume, and embryo percentage. Dominance effects were significant for oil content, oil index, seed index, and seed volume. Cytoplasmic and additive effects for parents and dominance effects in homozygous and heterozygous forms were predicted. Favorable genetic effects were predicted in this study and the results provided evidence that these seed traits can be genetically improved. In addition, chromosome associations with additive and dominance effects were detected and discussed in this study.

Last Modified: 8/30/2014
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