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United States Department of Agriculture

Agricultural Research Service

Research Project: MEASUREMENT SYSTEMS FOR DETERMINATION OF VITAMINS IN FOODS AND DIETARY SUPPLEMENTS Title: Simultaneous determination of water-soluble vitamins in SRM 1849 Infant/Adult Nutritional Formula powder by liquid chromatography–isotope dilution mass spectrometry

Authors
item Goldschmidt, Robert
item Wolf, Wayne

Submitted to: Analytical and Bioanalytical Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 7, 2009
Publication Date: January 10, 2010
Citation: Goldschmidt, R.J., Wolf, W.R. 2010. Simultaneous determination of water-soluble vitamins in SRM 1849 Infant/Adult Nutritional Formula powder by liquid chromatography–isotope dilution mass spectrometry. Analytical and Bioanalytical Chemistry. 397(2):471-481.

Interpretive Summary: Assessing dietary intake of vitamins from all sources, including foods, dietary supplements, and fortified foods, would be aided considerably by having analytical methodologies that are capable of simultaneous determination of several vitamins. Vitamins naturally present in foods may occur in different chemical forms, with levels ranging over several orders of magnitude. Vitamins in dietary supplements and fortified foods, however, are typically added in a single chemical form, where the influences of other materials present in addition to the vitamin are usually not as complex. Thus it should be relatively easy to determine multiple vitamins in these products simultaneously. Our recent work has focused on development of LC-UV/fluorescence and LC-MS/MS methods for the simultaneous determination of the water soluble vitamins thiamine, niacin, pyridoxine, pantothenic acid, folic acid, biotin, and riboflavin in dietary supplement tablets and fortified foods, such as formula powders and breakfast cereals. As part of the validation of these methods and of the collaboration to characterize a new NIST SRM 1849 Infant/Adult Nutritional Formula powder, we report data on SRM 1849 using isotope dilution mass spectrometric methods. Use of available NIST Standard Reference Materials® as test matrices in our method development and validation gives a benchmark for future application of these methods. We compared three chromatographic approaches and provide data on the stability of vitamin standard solutions for LC-based multiple vitamin determinations. This information will be useful to public and private sector laboratories which determine vitamin content in infant and adult liquid formulas.

Technical Abstract: Assessing dietary intake of vitamins from all sources, including foods, dietary supplements, and fortified foods, would be aided considerably by having analytical methodologies that are capable of simultaneous determination of several vitamins. Vitamins naturally present in foods may occur in different chemical forms, with levels ranging over several orders of magnitude. Vitamins in dietary supplements and fortified foods, however, are typically added in a single chemical form, and matrix issues are usually not as complex. These sources should thus be relatively amenable to approaches that aim for simultaneous determination of multiple vitamins. Our recent work has focused on development of LC-UV/fluorescence and LC-MS/MS methods for the simultaneous determination of the water soluble vitamins thiamine, niacin, pyridoxine, pantothenic acid, folic acid, biotin, and riboflavin in dietary supplement tablets and fortified foods, such as formula powders and breakfast cereals. As part of the validation of our methods and as part of the collaboration in characterization of a new NIST SRM 1849 Infant/Adult Nutritional Formula powder, we report data on SRM 1849 using isotope dilution-mass spectrometric methods. Use of available NIST Standard Reference Materials® as test matrices in our method development and validation gives a benchmark for future application of these methods. We compared three chromatographic approaches and provide data on stability of vitamin standard solutions for LC-based multiple vitamin determinations.

Last Modified: 10/19/2014
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