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United States Department of Agriculture

Agricultural Research Service

Research Project: EPIDEMIOLOGY, PATHOGENESIS AND COUNTERMEASURES TO PREVENT AND CONTROL ENTERIC VIRUSES OF POULTRY Title: Experimental inoculation of specific pathogen free broiler chickens with a thyroid homogenate, containing chicken astrovirus, which was collected from broiler chickens with runting-stunting syndrome

Authors
item Davis, James -
item Spackman, Erica
item Pantin-Jackwood, Mary
item Davis, Adam -

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: February 10, 2010
Publication Date: N/A

Technical Abstract: Thyroid glands were collected from field broiler chickens with clinical signs and lesions of Runting-Stunting Syndrome (RSS), submitted for histopathology and processed for polymerase chain reaction (PCR) testing using chicken Astrovirus primers. One-day-old White Rock specific pathogen free (SPF) chicks were inoculated, by the intraperitoneal route, with 0.1 ml of a homogenate produced from the thyroids of the field broilers, which was PCR positive. Chicks were screened for Astrovirus at 3, 7, & 10 days post-inoculation (DPI), and weighed at 3, 14, & 21 DPI. Radioimmunoassays for T3 were run on plasma samples. Results were compared with those of sham-inoculated controls.

Last Modified: 10/30/2014
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