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Submitted to: Biocontrol Science and Technology
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 1/26/2010 Publication Date: 2/19/2010 Citation: Allen, M.L., Walker III, W.B. 2010. Expressed Sequence Reference Standards for Evaluating Stage-specific Gene Expression in Southern Green Lacewings, Chrysoperla rufilabris. Biocontrol Science and Technology. 20: 6, 589-593, doi: 10.1080/09853151003657969. Interpretive Summary: The southern green lacewing, Chrysoperla rufilabris (Brumeister) is one of several generalist predators mass reared for sale to gardeners, horticulturists and vegetable producers in the United States. While these insects are quite amenable to mass rearing, they have not been studied as model organisms for genetic or many other research purposes. Thus, the availability of molecular tools for this organism is very limited. Molecular markers could be helpful in this insect, for studying its complex speciation, for estimating the overall health of mass-reared insects, and for identifying and understanding genes associated with insect predation. Five developmental stages, egg, young larva, mature larva, pupa, and adult, were tested using nine primer pairs. Three sequences were highly expressed at all life stages and six were differentially expressed. These primer pairs may be used as standards to quantitate functional gene expression associated with physiological processes such as reproduction, nutrition, and diapause. Technical Abstract: Five developmental stages of Chrysoperla rufilabris were tested using nine primer pairs. Three sequences were highly expressed at all life stages and six were differentially expressed. These primer pairs may be used as standards to quantitate functional gene expression associated with physiological processes such as reproduction, nutrition, and diapause. |
