|Gomez-Morales, M -|
|Ludovisi, A -|
|Pezzotti, P -|
|Amati, M -|
|Cherchi, S -|
|Lalle, M -|
|Pecoraro, F -|
|Pozio, E -|
Submitted to: Veterinary Parasitology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 15, 2009
Publication Date: December 23, 2009
Citation: Gomez-Morales, M.A., Ludovisi, A., Pezzotti, P., Amati, M., Cherchi, S., Lalle, M., Pecoraro, F., Pozio, E., Hill, D.E. 2009. International ring trial to detect anti-Trichinella IgG by ELISA on pig sera. Veterinary Parasitology. 166(3-4):241-248. Interpretive Summary: To determine the reproducibility and robustness of an ELISA to detect anti-Trichinella IgG in pig sera which was previously validated at the Community Reference Laboratory for Parasites (CRLP), a ring trial was organized involving European and extra-European reference laboratories for Trichinella.
Technical Abstract: In the present study, the sensitivity and specificity of the ELISA assay determined by the CRLP validation was 100% and 98.29%, respectively. The assay was reproducible, moreover, based on the receiver-operator characteristic (ROC) curve, the sensitivity and specificity of the assay reached 97.5% and 96.9%, respectively. The analysis of the differences in optical density (OD) between duplicates indicated a high repeatability of the ELISA with about 95% of the differences between -0.16 and 0.17 absorbance units. The accuracy of the test was determined by calculating the area under the ROC curve (AUC). Overall, the ELISA index (I(E)) showed a very high accuracy (AUC=0.9965) and it performed significantly better than the mean of the duplicated ODs (AUC=0.9387). The assay proposed is robust and reproducible and can be used for monitoring the lack of Trichinella infection in domestic pigs.