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United States Department of Agriculture

Agricultural Research Service

Title: Cloning and characterization of alpha-glucuronidase enzymes from mixed cultures

Authors
item Lee, Charles
item Kibblewhite, Rena
item Wagschal, Kurt
item Orts, William

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 4, 2010
Publication Date: April 19, 2010
Citation: Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzymes from mixed cultures. Meeting Abstract. 85.

Technical Abstract: Hemicellulose is second to cellulose as the most common carbohydrate source on the planet. Efficient utilization of this resource is essential to the economic viability of biomass refineries. Xylan, a primary component of hemicellulose, is a polymer of beta-1,4-linked xylose sugars. This polymer is targeted by endoxylanase and beta-xylosidase enzymes which will hydrolyze the substrate to simple sugar monomers for further downstream fermentation. However, the enzyme accessibility to the xylan is blocked by various chemical substituents. One of these chemical moieties is glucuronic acid which is found in the xylan of many plant tissues. The presence of the glucuronic acid substituent not only impairs enzymatic hydrolysis, but also stabilizes the xylosidic bonds against acid hydrolysis. The enzyme responsible for removing the glucuronic acid moiety is alpha-glucuronidase. We have employed a high throughput enzyme activity assay to screen mixed culture DNA libraries for new alpha-glucuronidase genes. We have cloned and biochemically characterized the corresponding enzymes. We will describe the unique activity profiles of the enzymes and their ability to enhance xylan degradation.

Last Modified: 12/18/2014
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