Location: Aquatic Animal Health Research
Title: Identification and expression profile of multiple genes in channel catfish fry after modified live Flavobacterium columnare vaccination Authors
|Wei Pridgeon, Yuping|
Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: January 26, 2010
Publication Date: January 27, 2010
Citation: Pridgeon, Y.W., Klesius, P.H. 2010. Identification and expression profile of multiple genes in channel catfish fry after modified live Flavobacterium columnare vaccination. Genbank. Accession Nos. GW392457 to GW392488. Technical Abstract: Using PCR-select subtractive cDNA hybridization technique, 32 expressed sequence tags (ESTs) were isolated from 96 clones of a channel catfish (Ictalurus punctatus) fry subtractive library ten minutes post vaccination with a modified live Flavobacterium columnare vaccine. The transcription levels of the 32 ESTs in response to F. columnare vaccination were then evaluated by quantitative PCR (QPCR). Of the 32 ESTs, 28 were upregulated in at least one vaccinated fish. Of the 28 upregulated ESTs, 12 were consistently induced at least 2 fold higher in vaccinated fish compared to unvaccinated control fish. Of the 12 upregulated genes, three (triglyceride lipase, PIKK family atypical protein kinase, and CCR4-NOT transcription complex subunit 1) were consistently upregulated greater than 3 fold. The 12 consistently upregulated genes also included CD59, polymerase (RNA) I polypeptide C, pyrophosphatase (inorganic) 1, mannose-P-dolichol utilization defect 1, Nascent polypeptide-associated complex subunit alpha, hemoglobin-beta, fetuin-B, glyoxalase domain containing 4, and putative histone H3. The 28 upregulated ESTs represent genes with putative functions in the following five major categories: (1) immune response (46%); (2) signal transduction (21%); (3) transcriptional regulation (11%); (4) cell maintenance (11%); and (5) unknown (11%).