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United States Department of Agriculture

Agricultural Research Service

Research Project: VALIDATION OF THE EFFECT OF INTERVENTIONS AND PROCESSES ON PERSISTENCE OF PATHOGENS ON FOODS Title: Challenges of microarray applications for microbial detection and gene expression profiling in food

Author
item Liu, Yanhong

Submitted to: Journal of Microbial and Biochemical Technology
Publication Type: Review Article
Publication Acceptance Date: June 18, 2011
Publication Date: June 20, 2011
Citation: Liu, Y. 2011. Challenges of microarray applications for microbial detection and gene expression profiling in food. Journal of Microbial and Biochemical Technology. Available: http://dx.doi.org/10.4172/1948-5948.S2-001.

Technical Abstract: Microarray technology represents one of the latest advances in molecular biology. The diverse types of microarrays have been applied to clinical and environmental microbiology, microbial ecology, and in human, veterinary, and plant diagnostics. Since multiple genes can be analyzed simultaneously, this technology has been extended to food microbiology for detection and gene expression analyses of food-borne pathogens. Although this technique has multiple applications, microarray technology presently has low sensitivity and also suffers from problems such as reproducibility and reliability. This paper focuses on microarray applications for microbial detection and gene expression profiling of food-borne pathogens; including some of the challenges and key issues such as target nucleic acid isolation and selection of target DNA sequences.

Last Modified: 10/31/2014
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