Skip to main content
ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Livestock Bio-Systems » Research » Publications at this Location » Publication #252391
Title: Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study

Author
item MASOPUST, MARTIN - Institute For Animal Genetics - Czech Republic
item VYKOUKALOVA, ZUZANA - Mendel University
item KNOLL, ALES - Institute For Animal Genetics - Czech Republic
item BARTENSCHLAGER, HEINZ - Hohenheim University
item MILEHAM, ALAN - Genus
item DEEB, NADER - Genus
item Rohrer, Gary
item CEPICA, STANISLAV - Institute For Animal Genetics - Czech Republic

Submitted to: Molecular Biology Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/8/2010
Publication Date: 4/15/2011
Citation: Masopust, M., Vykoukalova, Z., Knoll, A., Bartenschlager, H., Mileham, A., Deeb, N., Rohrer, G.A., Cepica, S. 2011. Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study. Molecular Biology Reports. 38:2611-2617.

Interpretive Summary: Insulin receptor substrate (IRS) genes are important factors controlling growth and body composition. The gene for IRS4 is expected to map to a region of the pig genome on chromosome X where QTL for backfat have been identified. Therefore, we determined the complete coding sequence of the porcine IRS4 gene. The pig gene product shares 92% identity with human IRS4 and possesses the same important regions as the human protein. We detected single nucleotide polymorphisms (SNP) in the promoter region (96C

Technical Abstract: Using PCR and IPCR techniques we obtained a 4498 bp nucleotide sequence FN424076 encompassing the complete coding sequence of the porcine IRS4 gene and its proximal promoter. The 1269-amino acid porcine protein deduced from the nucleotide sequence shares 92% identity with the human IRS4 and possesses the same domains and Tyr(P) motifs as the human protein. We detected substitution FN424076:g.96C