Title: Molecular Characterization of Swine Manure Lagoon Microbial and Antibiotic Resistant Populations Authors
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 13, 2010
Publication Date: May 23, 2010
Citation: Brooks, J.P., McLaughlin, M.R. 2010. Molecular Characterization of Swine Manure Lagoon Microbial and Antibiotic Resistant Populations [abstract]. Proceedings American Society of Microbiology. #Q-084. Technical Abstract: Background: The differences in swine manure lagoon effluent based on differing management styles or approaches such as different stages of swine rearing determines the presence of variable antibiotic resistance determinants and functional microbial populations. These concerns determine the suitability of swine lagoon effluent for use on various forage or other crop applications and whether swine effluent is a potential source of foodborne bacterial pathogens. Methods: The aim of this study was to assess and compare the molecular characteristics of antibiotic resistance determinants and microbial constituents between swine lagoon microbial populations from sow (n=17), nursery (n=10), and finisher (n=10) farms in the southeastern United States. Total DNA was extracted from these three different types of swine farms and analyzed for the presence of 16S rRNA, antibiotic resistant determinants, Salmonella, Campylobacter, and Listeria via RTiPCR. Fingerprints based on amplified ribosomal DNA restriction analyses (16S rRNA) were developed from each lagoon type and compared and used to determine clone library analyses. Results: Statistical analyses indicated that farm type influenced the presence of microbial populations, antibiotic resistant determinants and 16S rRNA genomic units. Typical resistance genetic determinants were related to tetracycline and beta-lactam resistance and were determined by swine farm type and confirmed previous cultural findings using the Kirby-Bauer antibiotic disc-diffusion technique. Conclusions: The presence of variable swine management styles and rearing influences the microbial constituents and antibiotic resistance profiles of swine manure lagoons.