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United States Department of Agriculture

Agricultural Research Service

Research Project: EPIDEMIOLOGY, PATHOGENESIS AND COUNTERMEASURES TO PREVENT AND CONTROL ENTERIC VIRUSES OF POULTRY

Location: Endemic Poultry Viral Diseases Research Unit

Title: Ultra high-throughput nucleic acid sequencing as a tool for virus discovery in the turkey gut.

Authors
item DAY, JAMES
item ZSAK, LASZLO

Submitted to: Feedinfo News Service
Publication Type: Trade Journal
Publication Acceptance Date: April 16, 2010
Publication Date: April 19, 2010
Citation: Day, J.M., Zsak, L. 2010. Ultra high-throughput nucleic acid sequencing as a tool for virus discovery in the turkey gut. Available: http:www.feedinfo.com.

Technical Abstract: Recently, the use of the next generation of nucleic acid sequencing technology (i.e., 454 pyrosequencing, as developed by Roche/454 Life Sciences) has allowed an in-depth look at the uncultivated microorganisms present in complex environmental samples, including samples with agricultural importance. This new technology allows researchers to gather and analyze a large amount of nucleic acid sequence data for a specific organism (genomics) or to compile sequence information for the multiple organisms present in a more complex sample (metagenomics). We have recently applied high-throughput pyrosequencing to prepare a turkey gut RNA virus metagenome and to discover known and novel RNA viruses in the turkey gut, specifically from turkey farms and flocks that have experienced a historically high incidence of enteric signs and performance problems.

Last Modified: 8/27/2014
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