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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR AND GENETIC MECHANISMS OF FUNGAL DISEASE RESISTANCE IN GRAIN CROPS Title: Gene Encoding a C-Type Cyclin in Mycosphaerella Graminicola is Involved in Mycelium Formation, Melanin Biosynthesis, Stress Response, and Pathogenicity

Authors
item Choi, Yoon-E
item Goodwin, Stephen

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: April 15, 2010
Publication Date: August 7, 2010
Citation: Choi, Y., Goodwin, S.B. 2010. Gene Encoding a C-Type Cyclin in Mycosphaerella Graminicola is Involved in Mycelium Formation, Melanin Biosynthesis, Stress Response, and Pathogenicity. Phytopathology.

Technical Abstract: Mycosphaerella graminicola is an important wheat pathogen causing septoria tritici blotch. To date, an efficient strategy to control M. graminicola has not been developed. More significantly, we have a limited understanding of the molecular mechanisms of M. graminicola pathogenicity. In this study, we attempted to characterize an MCC1-encoding c-type cyclin, a homologous gene to FCC1 in Fusarium verticillioides. Four independent MCC1 knock-out mutants were generated via Agrobacterium-mediated transformation (ATMT). All of the MCC1 mutants showed consistent multiple phenotypes. We could observe significant reductions in radial growth on PDA in all of the MCC1 mutants. In addition, MCC1 gene deletion mutants produced less mycelium on PDA, had increased melanin biosynthesis on agar plates, showed an increase in their stress tolerance response, and were reduced significantly in pathogenicity. These results indicate that the MCC1 gene is involved in multiple signaling pathways including pathogenicity in M. graminicola.

Last Modified: 12/18/2014
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