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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR AND GENETIC MECHANISMS OF FUNGAL DISEASE RESISTANCE IN GRAIN CROPS Title: Nutrient Acquisition and Generation of Reactive Oxygen Species Via CREA, AREA, and NOXa are Important in Pathogenicity in Mycosphaerella Graminicola

Authors
item Choi, Yoon-E
item Goodwin, Stephen

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: April 15, 2010
Publication Date: August 7, 2010
Citation: Choi, Y., Goodwin, S.B. 2010. Nutrient Acquisition and Generation of Reactive Oxygen Species Via CREA, AREA, and NOXa are Important in Pathogenicity in Mycosphaerella Graminicola. Phytopathology.

Technical Abstract: Mycosphaerella graminicola is an important wheat pathogen causing significant economic loss. M. graminicola is a hemibiotroph, indicating that a biotrophic stage with nutrient uptake and a necrotrophic stage associated with a possible toxin or reactive oxygen species (ROS) are important to pathogenicity. To better understand the pathogenic mechanisms of M. graminicola, we employed over-expression strategies; selected target genes for over-expression were CREA, AREA, and NOXa, which might function as regulators in nutrient acquisition and ROS generation. Increased expressions of CREA, AREA, and NOXa were confirmed via q-RT PCR and subsequently used for pathogenicity testing. Among them, the NOXa over-expression strain, NO2, resulted in significantly increased pathogenicity. Moreover, instead of the usual filamentous growth, we observed a significant predominance of yeast-like growth in NO2, which is correlated with ROS production. Our data indicate that ROS generation via NOXa is important to pathogenicity as well as development in M. graminicola.

Last Modified: 12/22/2014
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