ARS | Publication request: Development of core SSR markers for Gossypium germplasm characterization

Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Abstract Only
Publication Acceptance Date: December 10, 2009
Publication Date: April 19, 2010
Citation: Yu, J., Fang, D.D., Ulloa, M., Percy, R.G., Kohel, R.J., Hinze, L.L., Frelichowski, J.E., Cho, J., Campbell, B.T., Chee, P., Zhang, J., Abdurakhmonov, I., Abdukarimov, A., Jones, D.C. 2010. Development of core SSR markers for Gossypium germplasm characterization [abstract]. In: Proceedings of the National Cotton Council Beltwide Cotton Conferences, January 4-7, 2010, New Orleans, Louisiana. p. 811. CDROM.

Technical Abstract: A set of 105 portable DNA markers were carefully developed to provide a common basis for systematic characterization of cotton germplasm collections in the U.S. and throughout the world. The 105 PCR-based SSR markers of different origins were evenly distributed on each of the 26 cotton chromosomes with every chromosome arm having 2 markers at approximately 30 cM intervals. Each of these markers was examined on a standardized germplasm panel consisting of 12 diverse Gossypium genotypes. This set of DNA markers is presented to serve as an initial set of core DNA markers for global germplasm characterization. The initial core set can be modified and expanded as new DNA markers such as SNP markers are developed, characterized, and located in the cotton genome.