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United States Department of Agriculture

Agricultural Research Service

Research Project: Management of Off-flavors and Undesirable Bacteria in Aquaculture

Location: Natural Products Utilization Research

Title: Evaluation of free radical-generating compounds for toxicity towards the cyanobacterium Planktothrix perornata which causes musty off-flavor in pond-raised channel catfish (Ictalurus punctatus)

Authors
item Nanayakkara, N.P. Dhammika -
item Schrader, Kevin

Submitted to: Aquaculture Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 18, 2010
Publication Date: November 16, 2011
Citation: Nanayakkara, N., Schrader, K. 2011. Evaluation of free radical-generating compounds for toxicity towards the cyanobacterium Planktothrix perornata which causes musty off-flavor in pond-raised channel catfish (Ictalurus punctatus). Aquaculture Research. 42:1895-1898.

Interpretive Summary: Several compounds were evaluated for their selective toxicities towards a species of blue-green algae that produces the compound causing musty off-flavor in pond-raised channel catfish. Two of these compounds were very toxic towards the musty-odor blue-green algae but were not very toxic towards a beneficial species of green algae that also grows in catfish production ponds.

Technical Abstract: The cyanobacterium Planktothrix perornata grows in channel catfish (Ictalurus punctatus) production ponds in the southeastern United States and produces the musty-odor compound 2-methylisoborneol (MIB). MIB can rapidly accumulate in the flesh of the catfish, thereby rendering the fish unpalatable and unmarketable. A group of compounds that produce reactive oxygen intermediates (ROI) were evaluated using a rapid bioassay for their selective toxicities towards P. perornata. The most selectively toxic compounds were tert-butyl hydroperoxide and the 8-aminoquinoline derivative (-)-(R)-8-[(4-amino-1-methylbutyl)amino]-6-methoxy-4-methyl-5-[3,4-dichlorophenoxy]quinoline succinate], with lowest-complete-inhibition concentrations (LCIC) of 0.01 µM and 1.0 µM, respectively. The 8-aminoquinoline derivative and tert-butyl hydroperoxide are not likely candidates to pursue for efficacy studies in catfish production ponds due to their respective high manufacturing cost and toxicity profile. However, this study demonstrates that ROI-producing compounds are a priority target group for evaluation of selective toxicity towards P. perornata as the first step in the discovery of novel cost-effective, environmentally safe algaecides.

Last Modified: 10/25/2014
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