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United States Department of Agriculture

Agricultural Research Service

Research Project: MINIMIZING THE ADVERSE HEALTH AND ECONOMIC IMPACTS OF MYCOTOXINS AND PLANT TOXINS IN FOODS

Location: Toxicology and Mycotoxin Research

Title: Chemical and Toxicological Fate of Fumonisin B1 during Extrusion Processing of Corn Grits

Authors
item Jackson, Lauren -
item Jablonski, Joseph -
item Voss, Kenneth
item Hanna, Milford -
item Bianchini, Andreia -
item Bullerman, Lloyd -
item Ryu, Dojin -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 2010
Publication Date: June 7, 2010
Citation: Jackson, L.S., Jablonski, J., Voss, K.A., Hanna, M.A., Bianchini, A., Bullerman, L.B., Ryu, D. 2010. Chemical and Toxicological Fate of Fumonisin B1 during Extrusion Processing of Corn Grits. Fungi in Food and Beverages:New Research on Spoilage, Mycotoxins and Prevention. Jun 7-9,2010. Freisling, Germany.

Technical Abstract: Two batches of flaking corn grits were prepared by growing Fusarium verticillioides to contain low and high levels of fumonisin B1 (FB1), Batch-1 at 9.7 ppm and Batch-2 at 50 ppm FB1 as determined by HPLC. These two batches were extruded (Batch-1E; Batch-2E) or extruded with 10% w/w glucose supplementation (Batch-1EG; Batch-2EG) using a twin-screw extruder. FB1 concentrations after extrusion were: Batch-1E = 2.7 ppm; Batch-1EG = 0.6 ppm; Batch-2E = 18 ppm; and Batch-2EG = 5.7 ppm. These values corresponded to FB1 reductions of 72% (Batch-1E), 94% (Batch-1EG), 64% (Batch-2E) and 89% (Batch-2EG). Levels of N-(1-Deoxy-D-fructos-1-yl) FB1 ranged 1.9-2.4 % and 38-46% of total FB1 species when corn grits were extruded without glucose and with added glucose, respectively. Hydrolyzed fumonisin B1 was a minor species representing <15% of the total FB1 species present. In toxicological evaluation with rats, relative kidney weights were decreased in groups fed the Batch-1 or Batch-2 diets while apoptotic tubule lesions were found from animals fed the Batch-1, Batch-1E, Batch-2, Batch-2E, or Batch-2EG diets. The severity of the kidney lesions was in correspondence with the FB1 concentration in the diet. Overall, glucose supplementation enhanced FB1 reductions and in vivo toxicity of the extruded corn grits.

Last Modified: 9/1/2014
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