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United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT AND GENETIC CHARACTERIZATION OF AGRICULTURAL AND BIOTECHNOLOGICAL MICROBIAL RESOURCES

Location: Bacterial Foodborne Pathogens & Mycology Research Unit

Title: Kroppenstedtia eburnea gen. nov., sp. nov., a novel thermoactinomycete isolated by environmental screening, and emended description of the family Thermoactinomycetaceae Matsuo et al. 2006 emend. Yassin et al. 2009

Authors
item Von Jan, Mathias -
item Riegger, Nicole -
item Potter, Gabriele -
item Schumann, Peter -
item Verbarg, Susanne -
item Sproer, Cathrin -
item Rohde, Manfred -
item Lauer, Bettina -
item LABEDA, DAVID
item Klenk, Hans-Peter -

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 22, 2010
Publication Date: September 20, 2011
Citation: Von Jan, M., Riegger, N., Potter, G., Schumann, P., Verbarg, S., Sproer, C., Rohde, M., Lauer, B., Labeda, D.P., Klenk, H. 2011. Kroppenstedtia eburnea gen. nov., sp. nov., a novel thermoactinomycete isolated by environmental screening, and emended description of the family Thermoactinomycetaceae Matsuo et al. 2006 emend. Yassin et al. 2009. International Journal of Systematic and Evolutionary Microbiology. 61(9):2304-2310.

Interpretive Summary: During the course of environmental screening at a contract manufacturing facility in Southern Germany, a novel microorganism was isolated from testing of a surface sample. This microorganism was subjected to a thorough examination of its genetic, biochemical, morphological and physiological properties in an attempt to determine its identity. The resulting data conclusively demonstrated that the strain represented a species of new genus of thermophilic bacteria for which the name Kroppenstedtia eburnea is proposed. The formal description of this microorganism and its characteristics will permit other scientists throughout the world to recognize similar or related strains isolated from other environments and thus determine the ecology and role of this genus in nature.

Technical Abstract: A Gram-positive, spore-forming, aerobic, filamentous bacterium, strain JFMB-ATET, was isolated in 2008 during environmental screening on a surface in grade C in a contract manufacturing organization in southern Germany. The isolate grew at temperatures of 25-50°C and at pH 5.0-8.5, forming ivory-colored colonies with sparse white aerial mycelia. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the family Thermoactinomycetaceae, except that the cell-wall peptidoglycan contained LL-diaminopimelic acid, while all previously described members of this family display this diagnostic diamino acid in the meso-conformation. The DNA G + C content of the novel strain was 54.6 mol%, the main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, and the major menaquinone was MK-7. The major fatty acids had saturated C14 -C16 branched-chains. No diagnostic sugars were detected. Based on the chemotaxonomic results and 16S rRNA gene sequence analysis, the isolate is proposed to represent a novel genus and species, Kroppenstedtia eburnaea. The type strain is JFMB-ATET (= DSM 45196T = NRRL B-24804T = CCUG 59226T).

Last Modified: 7/28/2014
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