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United States Department of Agriculture

Agricultural Research Service

Research Project: NEWCASTLE DISEASE EPIDEMIOLOGY, PATHOGENESIS, AND CONTROL Title: Characterization of Newcastle disease virus isolates recovered from pigeons in the territory of the Russian Federation

Authors
item Pchelkina, Inna -
item Manin, Timofey -
item Kolosov, Sergey -
item Irza, Victor -
item Drygin, Vladimir -
item Starov, Sergey -
item Miller, Patti

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 2011
Publication Date: May 15, 2011
Citation: Pchelkina, I.P., Manin, T.B., Kolosov, S.N., Irza, V.N., Drygin, V.V., Starov, S.K., Miller, P.J. 2011. Characterization of Newcastle disease virus isolates recovered from pigeons in the territory of the Russian Federation [abstract]. 1st International Avian Respiratory Disease Converence, May 15-18, 2011, Athens, Georgia. p. 57.

Technical Abstract: Newcastle disease (ND) is a continual problem for the poultry industry with synanthropic birds representing one of the possible reservoirs of infection. Outbreaks of ND are regularly confirmed among pigeons in different regions of the Russian Federation. The spread of Newcastle disease virus (NDV) among pigeons occurs mainly in markets, exhibitions, in cases of personal contacts between pigeon breeders; the virus also spreads among both wild and synanthropic pigeons. Due to the threat associated with pigeons infecting poultry species, primarily Galliformes, with columbine NDV isolates, these viruses require further investigation. In 2009-2010, a number of NDV isolates were recovered from the organs of pigeons presenting with neurological signs (torticollis, ataxia) and high mortality rates. Necropsies demonstrated congestion and hemorrhages in brain tissue, along with lung edema and pneumonia. All NDV isolates recovered from the pigeon samples were studied using polymerase chain reaction (RT-PCR), and the sequences of Fusion (F) gene fragments were determined, including the nucleotide sequence which codes for the F-protein cleavage site. The isolates had cleavage site structure of virulent type: 112K-R-Q-K-R-F117. The ICPI values were above 0.7, also suggesting virulent properties for chickens. The comparative analysis of F-gene nucleotide sequences demonstrated that the studied NDV isolates belonged to genotype VI. They were 97-100% similar to each other and 94-98% similar to the isolates recovered from pigeons in Austria in 2000 (AY471789) (Aldous et al., 2003, 2004) and in Croatia in 2001 (AY150162) (Ujvari et al., 2003). However, they were only 95% similarity to typical NDV isolates from pigeons classified as the VIb subtype. Thus, results of the analysis of sequences of isolates recovered from pigeons in the RF territory in 2009-2010 indicate that they belong to relatively poorly studied genetic subline VIb/2 (Ujvari et al., 2003).

Last Modified: 11/28/2014
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