Title: Development and characterization of a LaSota strain recombinant Newcastle disease virus expressing the red fluorescent protein for use in co-infection studies Authors
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 2011
Publication Date: May 15, 2011
Citation: Li, J., Hu, H., Yu, Q., Miller, P.J. 2011. Development and characterization of a LaSota strain recombinant Newcastle disease virus expressing the red fluorescent protein for use in co-infection studies [abstract]. 1st International Avian Respiratory Disease Conference, May 15-18, 2011, Athens, Georgia. p. 53. Technical Abstract: Newcastle disease virus (NDV) infections result in significant economic losses to poultry producers around the world. The LaSota and B1 vaccine strains of NDV are commonly used to prevent the losses from Newcastle disease. Recombination of NDV is thought to occur based on the genomes of NDV isolates that appear to be mixtures of more than one NDV. To assess the potential of NDV isolates to recombine, two different NDV isolates must occupy the same cell at the same time. In the present study, we sought to develop a LaSota recombinant (rLS) NDV expressing red fluorescent protein (RFP), as a reporter gene that we could use to co-infect cell cultures, along with another recombinant NDV containing green fluorescent protein (GFP), rB1-GFP. To achieve this goal, we constructed a NDV LaSota strain complementary deoxyribonucleic acid (cDNA) clone, inserted the RFP gene, and rescued an infectious, recombinant NDV LaSota virus by using reverse genetics technology. The appearance of RFP in live infected cells will confirm the recovery of a recombinant NDV (rLS-RFP) expressing the reporter gene. The recombinant virus, rLS-RFP, will be characterized by mean death time (MDT), intracerebral pathogenicity index (ICPI) and growth curve assays. The recombinants, rLS-RFP and rB1-GFP, will be used to co-infect chicken embryo kidney (CEK), DF1, and Vero cells.