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United States Department of Agriculture

Agricultural Research Service

Research Project: ALLIUM, CUCUMIS, AND DAUCUS GERMPLASM ENHANCEMENT, GENETICS, AND BIOCHEMISTRY

Location: Vegetable Crops Research Unit

Title: A linkage map of cultivated cucumber (cucumis sativus l.) with 248 microsatellite marker loci and seven genes for horticulturally important traits

Authors
item Miao, Han -
item Zhang, Shengping -
item Wang, Xiaowu -
item Zhang, Zhonghua -
item Li, Man -
item Mu, Shengqi -
item Cheng, Zhouchao -
item Zhang, Ruowei -
item Huang, Sanwen -
item Xie, Bingyan -
item Fang, Zhiyuan -
item Zhang, Zhenxian -
item Weng, Yiqun
item Gu, Xingfang -

Submitted to: Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 15, 2011
Publication Date: April 16, 2011
Citation: Miao, H., Zhang, S., Wang, X., Zhang, Z., Li, M., Mu, S., Cheng, Z., Zhang, R., Huang, S., Xie, B., Fang, Z., Zhang, Z., Weng, Y., Gu, X. 2011. A linkage map of cultivated cucumber (cucumis sativus l.) with 248 microsatellite marker loci and seven genes for horticulturally important traits. Euphytica. Available: http://www.springerlink.com/content/l8u2603qg4657g53/fulltext.html.

Interpretive Summary: A genetic map was developed with microsatellite (simple sequence repeat, SSR) markers and 148 recombinant inbred lines (RILs) derived from a cross between two cultivated cucumber (Cucumis sativus L.) inbred lines 9110Gt and 9930, which was also segregating for seven horticulturally important traits including bitterfree foliage (bi), gynoecious sex expression (F), uniform immature fruit color (u), glossy fruit skin (d), heavy netting of mature fruit (H), no fruit ribbing (fr), and virescent leaf (v-1). Linkage analysis placed 248 microsatellite loci into seven linkage groups spanning 711.9 cM with a mean marker interval of 2.8 cM. Based on shared markers with an early cucumber genetic map, the 7 linkage groups could be assigned to seven cucumber chromosomes. The four fruit epidermal feature-related genes, u, d, H and fr were found to be tightly linked loci in chromosome 5, and the other three (F, bi and v-1) were placed in different locations of chromosome 6. Except for gene F, it was the first time for all other six genes to be mapped with molecular markers. In addition, this is the first report of the inheritance of fruit ribbing in cucumber, which was controlled by a single, dominant gene designated as Fr. Mapping information from this study opens the way for marker-assisted selection and map-based cloning of these horticulturally important genes in cucumber.

Technical Abstract: Marker assisted selection (MAS) is playing an increasingly important role in expedite and increase the efficiency of classical plant breeding. In cucumber, MAS is lagging behind as compared with other field crops. In the present study, a genetic map was developed with microsatellite (or simple sequence repeat, SSR) markers and 148 recombinant inbred lines (RILs) derived from a cross between two cultivated cucumber inbred lines 9110Gt and 9930, which was also segregating for seven horticulturally important traits including bitterfree foliage (bi), gynoecious sex expression (F), uniform immature fruit color (u), glossy fruit skin (d), heavy netting of mature fruit (H), no fruit ribbing (fr), and virescent leaf (v-1). Linkage analysis placed 248 microsatellite loci into seven linkage groups (chromosomes) spanning 711.9 cM with a mean marker interval of 2.8 cM. The four fruit epidermal feature-related genes, u, d, H and fr were found to be tightly linked loci in chromosome 5, and the other three (F, bi and v-1) were placed in different locations of chromosome 6. Except for gene F, it was the first time for all other six genes to be mapped with molecular markers. In addition, this is the first report of the inheritance of fruit ribbing in cucumber, which was controlled by a single, dominant gene designated as Fr. Mapping information from this study opens the way for marker-assisted selection and map-based cloning of these horticulturally important genes in cucumber.

Last Modified: 4/23/2014
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