Technical Abstract: Plant stress significantly reduces plant productivity. Automated on-the-go mapping of plant stress would allow for a timely intervention and mitigation of the problem before critical thresholds are exceeded, thereby maximizing productivity. The spectral signature of plant leaves was analyzed by a hyperspectral camera to identify the onset and intensity of plant water stress. Five different levels of water treatment were created in young apple trees (cv. 'Buckeye Gala') in a greenhouse. The trees were periodically monitored with a hyperspectral camera along with an active-illuminated spectral vegetation sensor and a digital color camera. Individual spectral images over a 400 - 1000 nm wavelength range were extracted at a specific wavelength to estimate reflectance and generate spectral profiles for the five different water treatment levels. Various spectral indices were calculated and correlated to stress levels. The highest correlation was found with Red Edge NDVI at 705 nm and 750 nm in narrowband indices and NDVI at 680 nm and 800 nm in broadband indices. The experimental results indicated that intelligent optical sensors could deliver decision support for plant stress detection and management.