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United States Department of Agriculture

Agricultural Research Service

Research Project: ALLIUM, CUCUMIS, AND DAUCUS GERMPLASM ENHANCEMENT, GENETICS, AND BIOCHEMISTRY Title: Fine mapping of paternal sorting of mitochondria (psm) in cucumber

Authors
item Park, Young -
item Calderon, Claudia -
item Al-Faifi, Sulieman -
item Havey, Michael

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 1, 2011
Publication Date: November 5, 2011
Citation: Park, Y.H., Calderon, C., Al-Faifi, S., Havey, M.J. 2011. Fine mapping of paternal sorting of mitochondria (psm) in cucumber [abstract]. 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference. Paper No. OP9-1.

Technical Abstract: Cucumber is unique among plants because its mitochondrial DNA shows paternal transmission, is one of the largest known among all plants, due largely to short repetitive DNA motifs, and undergoes recombination among repeats to produce rearranged mitochondrial DNAs associated with strongly mosaic (MSC) phenotypes. We undertook a germplasm screen to identify cucumber accessions which produced relatively large frequencies of wild-type progenies when crossed with MSC plants as the male. We identified a unique nuclear locus (Psm) which controls sorting of wild-type versus MSC mitochondria transmitted by the male gametophyte. A large segregating family was produced and F2 plants were crossed as the female with MSC16. Over 450 testcross families were scored for numbers of wild-type versus MSC progenies and molecular markers, assigning Psm to a 2.7 cM region on chromosome 3. Eventual cloning of Psm should provide unique insights about nuclear-mitochondrial interactions that control the prevalence of specific mitochondrial DNAs.

Last Modified: 10/30/2014
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