Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 2011
Publication Date: June 24, 2011
Citation: Guo, B., Fedorova, C., Wang, W., Nierman, W., Chen, X., Bhatnagar, D., Yu, J. 2011. Gene expression profiling and identification of resistance genes to Aspergullus flavus infection in peanut through EST and microarray strategies. American Peanut Research and Education Society Abstracts. APRES meeting in San Antonio, TX. July 12-14, 2011. Technical Abstract: Aspergillus flavus and A. parasiticus infect peanut seeds and produce aflatoxins, which are associated with various diseases in domestic animals and humans throughout the world. The most cost-effective strategy to against aflatoxin contamination involves the development of peanut cultivars that are resistant to fungal infection and/or aflatoxin production. To identify peanut Aspergillus-interactive and Aspergillus-resistance genes, we carried out a large scale peanut Expressed Sequence Tag (EST) project followed by a peanut microarray construction. The fabricated microarray represents over 40% protein coding genes in the peanut genome. For expression profiling, resistant and susceptible peanut cultivars were infected with a mixture of A. flavus and parasiticus spores. Microarray analysis identified 65 and 1 genes in resistant and susceptible cultivars, respectively, that were up-regulated in response to Aspergillus infection. In addition we identified 40 putative Aspergillus-resistance genes that were constitutively up-expressed in the resistant cultivar in comparison to the susceptible cultivar. Some of these genes were homologous to peanut, corn, and soybean genes previously shown to confer resistance to fungal infection. This study is a first step towards a comprehensive genome-scale platform for developing Aspergillus-resistant peanut cultivars through targeted marker-assisted breeding and genetic engineering.