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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC IMPROVEMENT OF FRUIT CROPS THROUGH FUNCTIONAL GENOMICS AND BREEDING

Location: Appalachian Fruit Research Laboratory: Innovative Fruit Production, Improvement and Protection

Title: Transferability of apple EST-SSRs and assessment of genetic diversity in pear genotypes

Authors
item Zheng, Darman -
item Wu, Jun -
item Khan, Awais -
item BELL, RICHARD
item Korban, Schuyler -

Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 13, 2011
Publication Date: June 15, 2011
Citation: Zheng, D., Wu, J., Khan, A., Bell, R.L., Korban, S. 2011. Transferability of apple EST-SSRs and assessment of genetic diversity in pear genotypes [abstract]. American Society of Plant Biologists Annual Meeting. p. 15.

Technical Abstract: A total of 1,062 apple EST-derived simple sequence repeat (SSR) markers were screened and evaluated for cross-species transferability onto two different pear cultivars. PCR amplification of pear DNA leaf tissue samples was successful using 721 primer pairs demonstrating a high efficiency of transferability (67.9 percent) between apple and pear. Eight markers were selected to assess available genetic diversity in 48 genotypes of pear (Pyrus). A total of 140 gene loci were identified, of which 129 (92.14 percent) were polymorphic. Moreover, different pear cultivars could be readily distinguished. Based on polymorphisms revealed by the polymorphic SSR marker, an Unweighted Pair Group Method with Arithmetic mean (UPGMA) cluster analysis was performed using soft NTY-SIS-pc2.01. Pear genotypes were classified into two major classes and grouped based on their Asiatic and European origin into clusters.

Last Modified: 9/10/2014
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