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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVED PLANT GENETIC RESOURCES FOR PASTURES AND RANGELANDS IN THE TEMPERATE SEMIARID REGIONS OF THE WESTERN U.S.

Location: Forage and Range Research

Title: USDA, ARS European long greenhouse cucumber inbred backcross line population

Authors
item Staub, Jack
item Delannay, Isabelle -

Submitted to: HortScience
Publication Type: Germplasm Registration
Publication Acceptance Date: July 20, 2011
Publication Date: November 15, 2011
Citation: Staub, J.E., Delannay, I.Y. 2011. USDA, ARS European long greenhouse cucumber inbred backcross line population. HortScience. 46:1317-1320.

Interpretive Summary: A series of 116 European Long Greenhouse market type inbred backcross lines (IBL) were released in January 2011 by the Agricultural Research Service, United States Department of Agriculture. The IBL were developed by initially crossing an elite commercial European Long Greenhouse line and PI 432858 (China; long-fruited, Northern Chinese protected culture type), and with subsequent marker-assisted backcrossing and self-pollination. The IBL are available to U.S. cucumber breeders for developing long-fruited greenhouse market types suitable for protected-culture production (e.g., greenhouses and plastic tunnels) with increased genetic diversity and yield potential. These diverse IBL will be useful in genetic studies and/or to evaluate cross-progeny derived from crosses between IBL of this population and other long-fruited germplasm during the development of improved cucumber germplasm. The IBL were developed by crossing the elite commercial line NZ1 (Nunhems Vegetable Seeds, Haelen, The Netherlands) and PI 432858 (China; donor parent), and then backcrossing the most genetically diverse BC1 and BC2 progeny to the elite parent (cloned) as defined by marker analyses, followed by single seed descent without selection. At BC1, 30 of 288 individuals were selected (selection intensity = 11%) based on molecular market profiles [19 polymorphic, mapped simple sequence repeat (SSR) and sequence characterized amplified region (SCAR) marker loci] and then subsequently at the BC2, 120 of 384 individuals were chosen (selection intensity = 31%) based on their heterozygosity at 44 polymorphic markers [SSR, SCAR, single nucleotide polymorphism (SNP), expressed sequence tags (EST), and bacterial artificial chromosome (BAC-end)]. The selected BC2 individuals were then self-pollinated for three generations by single seed descent to generate 116 BC2S3 IBL. The morphological characteristics of these IBL were then evaluated in Madison, Wisconsin, and in Haelen and Bergschenhoek, The Netherlands under commercial greenhouse growing conditions.

Technical Abstract: A series of 116 European Long Greenhouse market type inbred backcross lines (IBL) were released in January 2011 by the Agricultural Research Service, United States Department of Agriculture. The IBL were developed by initially crossing an elite commercial European Long Greenhouse line and PI 432858 (China; long-fruited, Northern Chinese protected culture type), and with subsequent marker-assisted backcrossing and self-pollination. The IBL are available to U.S. cucumber breeders for developing long-fruited greenhouse market types suitable for protected-culture production (e.g., greenhouses and plastic tunnels) with increased genetic diversity and yield potential. These diverse IBL will be useful in genetic studies and/or to evaluate cross-progeny derived from crosses between IBL of this population and other long-fruited germplasm during the development of improved cucumber germplasm. The IBL were developed by crossing the elite commercial line NZ1 (Nunhems Vegetable Seeds, Haelen, The Netherlands) and PI 432858 (China; donor parent), and then backcrossing the most genetically diverse BC1 and BC2 progeny to the elite parent (cloned) as defined by marker analyses, followed by single seed descent without selection. At BC1, 30 of 288 individuals were selected (selection intensity = 11%) based on molecular marker profiles [19 polymorphic, mapped simple sequence repeat (SSR) and sequence characterized amplified region (SCAR) marker loci], and then subsequently at the BC2, 120 of 384 individuals were chosen (selection intensity = 31%) based on their heterozygosity at 44 polymorphic markers [SSR, SCAR, single nucleotide polymorphism (SNP), expressed sequence tags (EST), and bacterial artificial chromosome (BAC-end)]. The selected BC2 individuals were then self-pollinated for three generations by single seed descent to generate 116 BC2S3 IBL. The morphological characteristics of these IBL were then evaluated in Madison, Wisconsin, and in Haelen and Bergschenhoek, The Netherlands under commercial greenhouse growing conditions.

Last Modified: 9/1/2014
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