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United States Department of Agriculture

Agricultural Research Service

Research Project: FUNCTIONAL GENOMICS FOR IMPROVING NUTRIENTS AND QUALITY IN ALFALFA AND SOYBEAN

Location: Plant Science Research

Title: The transcriptome of common bean: nodules to beans

Authors
item O'Rourke, Jamie
item Iniguez, Luis -
item Bucciarelli, Bruna
item Woody, Jenna -
item Mcclean, Phillip -
item Jackson, Scott -
item Shoemaker, Randy
item Hernandez, Georgina -
item Vance, Carroll

Submitted to: International Congress of Biochemistry and Molecular Biology
Publication Type: Abstract Only
Publication Acceptance Date: September 21, 2011
Publication Date: November 19, 2011
Citation: O'Rourke, J.A., Iniguez, L.P., Bucciarelli, B., Woody, J.L., McClean, P.E., Jackson, S.A., Shoemaker, R.C., Hernandez, G., Vance, C.P. 2011. The transcriptome of common bean: nodules to beans [abstract]. XIV National Congress of Biochemistry and Plant Molecular Biology and 7th Symposium Mexico - USA, November 29-December 2, 2011, Campeche, Mexico. p. 17.

Technical Abstract: Phaseolus vulgaris (common bean) is one of the most important grain legumes for direct human consumption. It comprises 50% of the grain legumes consumed worldwide and is important as a primary source of dietary protein in developing countries. We performed next generation sequencing (RNAseq) on five tissues (seeds, pods, leaves, roots, and nodules) of Phaseolus vulgaris cv. Negro Jamapa plants that were inoculated with an effective rhizobium, an ineffective rhizobium mutant strain (fix-), or were provided nitrogen via fertilization. RNA was sequenced from several types of organs at various stages of development, generating 2.1x10**9 bp of sequence data. The sequences were used in a de-novo assembly to identify expressed transcripts. Our study identified 80,463 transcript assemblies (greater than or equal to 100bp). Of these, 1,175 were expressed specifically in seeds, 827 in pods, 3,054 in roots, and 1,686 in nodules, respectively. Comparing expression profiles of transcripts in the tissues has allowed us to identify those involved in nodule, root, pod, and seed development, symbiotic nitrogen fixation, and transcripts related to the functions of the tissues analyzed. This is the first whole plant transcriptome study of Phaseolus vulgaris.

Last Modified: 8/30/2014
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