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ARS Home » Pacific West Area » Pullman, Washington » Grain Legume Genetics Physiology Research » Research » Publications at this Location » Publication #275164

Title: Developing a method to scale up production of solanapyrone toxins by Ascochyta rabiei

Author
item KIM, WONGYONG - Washington State University
item Vandemark, George
item Chen, Weidong

Submitted to: Pisum Genetics
Publication Type: Abstract Only
Publication Acceptance Date: 4/5/2012
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Ascochyta rabiei, the causal agent of Ascochyta blight of chickpea, produces solanapyrone toxins. The toxins may play a role in pathogenesis. In order to develop toxin assays for screening chickpea genotypes and to investigate the role of the toxins in developing the disease Ascochyta blight, a method to scale up production of the solanapyrone toxins is needed. A new method with solid medium using natural substrates is being developed to replace the previously published method with liquid medium in static culture. The new method significantly improved production of the solaynapyrone toxins with much reduced requirement of laboratory space, and will facilitate future research employing and exploiting the solanapyrone toxins.