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United States Department of Agriculture

Agricultural Research Service

Research Project: Cool Season Grain Legume Genetic Enhancement and Pathology

Location: Grain Legume Genetics Physiology Research

Title: Comparison of transcriptomes between Sclerotinia sclerotiorum and S. trifoliorum using 454 Titanium RNA sequencing

Authors
item Qiu, Dan -
item Vandemark, George
item Chen, Weidong

Submitted to: Pisum Genetics
Publication Type: Abstract Only
Publication Acceptance Date: April 5, 2012
Publication Date: N/A

Technical Abstract: Sclerotinia sclerotiorum and S. trifoliorum cause Sclerotinia stem and crown rot of chickpea and white mold on many economically important crops. The host range of S. trifoliorum is mainly on cool season forage and grain legumes of about 40 plant species, whereas the host range of S. sclerotiorum encompasses more than 400 plant species including all the host plant species of S. trifoliorum. Despite morphological and ecological differences between the two species, both species are equally pathogenic on chickpea. Extensive research has been conducted on S. sclerotiorum and its genome sequences are available. However, relatively very little is known about S. trifoliorum. To take advantages of the genomic information of S. sclerotiorum, we compared the transcriptome of S. trifoliorum with that of S. sclerotiorum in order to gain a better understanding of the biology of both species. Total mRNAs of both species during vegetative growth were extracted and sequenced using the latest 454 Titanium RNA sequencing technology. A total of 23325 unique transcripts with average length of 534 nt (12.5 mb genome coverage) were obtained from S. sclerotiorum, whereas 21214 unique transcripts with average length of 509 nt (10.8 mb genome coverage) were obtained from S. trifoliorum. Comparison of the transcripts between the two species will be presented and their implications will be discussed.

Last Modified: 9/22/2014
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