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United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT AND GENETIC CHARACTERIZATION OF AGRICULTURAL AND BIOTECHNOLOGICAL MICROBIAL RESOURCES Title: Relationships among genera of the Saccharomycotina from multigene sequence analysis

Author
item Kurtzman, Cletus

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: August 30, 2012
Publication Date: August 30, 2012
Citation: Kurtzman, C.P. 2012. Relationships among genera of the Saccharomycotina from multigene sequence analysis [abstract]. International Congress on Yeasts.

Technical Abstract: Most known species of the subphylum Saccharomycotina (budding ascomycetous yeasts) have now been placed in phylogenetically defined clades following multigene sequence analysis. Terminal clades, which are usually well supported from bootstrap analysis, are viewed as phylogenetically circumscribed genera. However, relationships among many genera have either not been examined or the data have given only weak support for genus placement. Reported here is an analysis of the phylogeny of the Saccharomycotina based on concatenation of sequences from the following five genes: large and small subunit rRNAs, translation elongation factor 1-a, and RNA polymerases 1 and 2. The dataset was composed of type species from 70 currently accepted genera as well as species assigned to the genus Candida that are not members of known teleomorphic genera. Reference species included members of the Taphrinomycotina and Basidiomycota. Based on multigene analysis, all known ‘budding yeasts’ are members of the Saccharomycotina and the species were resolved into 12 major clades. In the context of assigned genera, the Saccharomycetaceae and the Debaryomycetaceae are the two largest families of the Saccharomycotina. As shown previously in smaller datasets, growth reactions on common media and ascospore morphology usually did not correlate with phylogenetic placement, but uncommon physiological properties, such as growth on methanol, purines, and pyrimidines strongly correlated with phylogeny.

Last Modified: 12/25/2014
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