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United States Department of Agriculture

Agricultural Research Service

Research Project: PATHOGEN DETECTION AND INTERVENTION METHODS FOR SHELLFISH

Location: Food Safety and Intervention Technologies

Title: Detection of enteric viruses in shellfish

Authors
item Richards, Gary
item Cliver, Dean -
item Greening, Gail -

Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: August 6, 2013
Publication Date: August 25, 2013
Citation: Richards, G.P., Cliver, D.O., Greening, G.E. 2013. Detection of enteric viruses in shellfish. Meeting Proceedings. G. Sauve, editor In Molluscan Shellfish Safety, Proceedings of the 8th International Conference on Molluscan Shellfish Safety, Charlottetown, Prince Edward Island, Canada, June 12-17, 2011.Springer, New York, pp. 177-183.

Technical Abstract: Norovirus and hepatitis A virus contamination are significant threats to the safety of shellfish and other foods. Methods for the extraction and assay of these viruses from shellfish are complex, time consuming, and technically challenging. Here, we itemize some of the salient points in extracting viruses from shellfish tissues and analyzing the extracts. A more comprehensive treatment of these topics will soon be available in: Richards, G. P., Cliver, D. O., & Greening, G. E. (in press). Foodborne viruses. Compendium of methods for the microbiological examination of foods, 5th ed., American Public Health Association, Washington, DC. Here, we describe some of the basic principles of virus extraction and molecular assay of viruses in shellfish, the importance of controls, and the limitations of both the extraction and assay methods. Recent efforts to validate methods for virus detection in shellfish center on a method currently in use in Canada and another method under validation in the European Union. The availability of such methods is important in tracking sources of outbreaks and may lead to the establishment of routine monitoring programs.

Last Modified: 11/26/2014
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