Location: Vegetable Crops Research Unit
Title: In vitro culture of cucumber microspores Authors
|Kielkowska, Agnieszka -|
Submitted to: Cucurbit Genetics Cooperative Report
Publication Type: Research Notes
Publication Acceptance Date: February 2, 2012
Publication Date: N/A
Technical Abstract: Our aim was to develop protocol for embryogenesis of cucumber microspores as possible targets for mitochondrial transformation. Protocols for successful isolation and viability testing of cultured microspores were identified. Mean percentage of viable microspores from in vitro cultured plants was low and reached about 18% on second day after isolation. During the first 7 days of culture we observed swelling of the microspores and displacement of the nucleus to a peripheral position. Heat and cold stresses did not induced microspore divisions. Although sterile cultures of uninucleate microspores were produced, no in vitro embryogenesis or maturation of microspores was observed due to meiotic abnormalities and decreased viability.