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United States Department of Agriculture

Agricultural Research Service

Research Project: Integrated Aquatic Animal Health Strategies

Location: Aquatic Animal Health Research

Title: Global gene expression in channel catfish after vaccination with an attenuated Edwardsiella ictaluri

Authors
item Wei Pridgeon, Yuping
item Yeh, Hung-Yueh
item Shoemaker, Craig
item Mu, Xingjiang -
item Klesius, Phillip

Submitted to: Fish and Shellfish Immunology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 28, 2011
Publication Date: February 16, 2012
Repository URL: http://handle.nal.usda.gov/10113/55457
Citation: Wei Pridgeon, Y., Yeh, H., Shoemaker, C.A., Mu, X., Klesius, P.H. 2012. Global gene expression in channel catfish after vaccination with an attenuated Edwardsiella ictaluri. Fish and Shellfish Immunology. 32:524-533.

Interpretive Summary: To understand the global gene expression in channel catfish after immersion vaccination with AquaVac ESC vaccine, microarray analysis were performed. A total of 52 unique genes were found to be up-regulated in vaccinated fish at 48h post vaccination, whereas a total of 129 were down-regulated. Microarray analysis revealed that apolipoprotein A-I was upregulated the most (8.5 fold, P = 0.011) at 48h post vaccination whereas a novel protein (accession no. CV995854 was down regulated the most (342 fold, P = 0.001). Our results suggest that these differentially regulated genes elicited by the vaccination might play important roles in protection of channel catfish against E. ictaluri.

Technical Abstract: To understand the global gene expression in channel catfish after immersion vaccination with an attenuated Edwardsiella ictaluri (AquaVac ESCTM), microarray analysis of 65,182 UniGene transcripts were performed. With a filter of false-discovery rate less than 0.05 and fold change greater than 2, a total of 52 unique transcripts were found to be upregulated in vaccinated fish at 48h post vaccination, whereas a total of 129 were downregulated. The 52 upregulated transcripts represent genes with putative functions in the following seven major categories: (1) hypothetical (25%); (2) novel (23%); (3) immune response (17%); (4) signal transduction (15%); (5) cell structure (8%); (6) metabolism (4%); and (7) others (8%). The 129 downregulated transcripts represent genes with putative functions in the following ten major categories: (1) novel (25%); (2) immune response (23%); (3) hypothetical (12%); (4) metabolism (10%); (5) signal transduction (7%); (6) protein synthesis (6.2%); (7) cell structure (5%); (8) apoptosis (3%); (9) transcription/translation (2%); and (10) others (6%). Microarray analysis revealed that apolipoprotein A-I was upregulated the most (8.5 fold, P = 0.011) at 48h post vaccination whereas a novel protein (accession no. CV995854) was downregulated the most (342 fold, P = 0.001). Differential regulation of several randomly selected transcripts in vaccinated fish was also validated by quantitative PCR. Our results suggest that these differentially regulated genes elicited by the vaccination might play important roles in the protection of channel catfish against E. ictaluri.

Last Modified: 9/1/2014
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