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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #280050
Title: The role of trichothecenes in the Triticeae-Fusarium graminearum interactions

Author
item MUEHLBAUER, GARY - University Of Minnesota
item BODDU, JAYANAND - University Of Minnesota
item GARDINER, STEPHANIE - University Of Minnesota
item SHIN, SANGHYUN - University Of Minnesota
item JIA, HAIYAN - University Of Minnesota
item CHO, SEUNGHO - University Of Minnesota
item McCormick, Susan
item SCHWEIGER, WOLFGANG - University Of Natural Resources & Applied Life Sciences - Austria
item LEMMONS, MARC - University Of Natural Resources & Applied Life Sciences - Austria
item BERTHILLER, FRANZ - University Of Natural Resources & Applied Life Sciences - Austria
item HAMETNER, CHRISTIAN - Vienna University Of Technology
item KOVALSKY PARIS, PAULA - University Of Natural Resources & Applied Life Sciences - Austria
item TORRES-ACOSTA, JUAN - University Of Natural Resources & Applied Life Sciences - Austria
item ADAM, GERHARD - University Of Natural Resources & Applied Life Sciences - Austria

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/8/2012
Publication Date: 8/8/2012
Citation: Muehlbauer, G.J., Boddu, J., Gardiner, S., Shin, S., Jia, H., Cho, S., Mccormick, S.P., Schweiger, W., Lemmons, M., Berthiller, F., Hametner, C., Kovalsky Paris, P.M., Torres-Acosta, J.A., Adam, G. 2012. The role of trichothecenes in the Triticeae-Fusarium graminearum interactions. American Phytopathological Society Abstracts.

Interpretive Summary:

Technical Abstract: Fusarium Head Blight (FHB), caused by Fusarium graminearum, is a major disease problem for the small grain crops wheat and barley. During infection, F. graminearum produces trichothecene mycotoxins such as deoxynivalenol (DON) that increase the aggressiveness of the fungus and reduces grain quality. Thus, we are interested in identifying genes that protect wheat and barley from the toxic effects of trichothecenes. Previous work had identified an Arabidopsis DOGT1 gene, encoding an UDP-glucosyltransferase (UGT), as involved in trichothecene resistance via conjugation of DON to DON-3-glucoside (D3G). We examined transcript profiles in wheat and barley during Fusarium graminearum infection and inoculation with the trichothecene deoxynivalenol (DON). From these experiments, we identified a set of 10 barley UDP-glucosyltransferases (UGT) that were upregulated during F. graminearum infection or DON treatment. We screened this set of UGTs in yeast and identified a barley UGT (HvUGT13248) gene that exhibited resistance to DON via conjugation to D3G. Transgenic Arabidopsis overexpressing HvUGT13248 exhibited the ability to grow on media containing DON. DON feeding studies on the transgenic Arabidopsis showed that DON was conjugated to D3G. In contrast to prior work in Arabidopsis overexpressing DOGT1 exhibited a dwarf phenotype due to brassinosteroid conjugation, transgenic Arabidopsis overexpressing HvUGT13248 did not exhibit dramatic changes in morphology and did not conjugate the brassinosteroid castasterone. More recently, we developed transgenic wheat overexpressing HvUGT13248 and showed that these lines exhibited high type II FHB resistance.