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United States Department of Agriculture

Agricultural Research Service

Research Project: GENETIC AND BIOLOGICAL DETERMINANTS OF AVIAN TUMOR VIRUS PATHOGENICITY, TRANSMISSION, AND EVOLUTION

Location: Avian Disease and Oncology Laboratory

Title: Use of Polymerase chain reaction (PCR) in diagnosis of Marek’s disease and reticuloendotheliosis in formalin-fixed, paraffin-embedded tumorous tissues

Authors
item Fadly, Aly
item Cao, Weisheng -
item Fulton, Richard -
item Mays, Jody
item Dunn, John
item Silva, Robert

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 11, 2012
Publication Date: August 5, 2012
Citation: Fadly, A.M., Cao, W., Fulton, R.M., Mays, J.K., Dunn, J.R., Silva, R.F. 2012. Use of Polymerase chain reaction (PCR) in diagnosis of Marek’s disease and reticuloendotheliosis in formalin-fixed, paraffin-embedded tumorous tissues. Meeting Abstract. Paper No. 12498.

Technical Abstract: PCR was used in diagnosis of Marek’s disease (MD) and reticuloendotheliosis (RE) in formalin-fixed, paraffin-embedded (FFPE) tumorous tissues that have been stored for periods varied from 5-244 months. In another experiment, PCR was also used in diagnosis of MD in tumorous tissues that have been only preserved in formalin for periods that varied from 7-49 days. MD and RE were detected in FFPE tissues tested even in those stored for up to 20+ years; MD was also detected in tissues preserved in formalin for up to 7 weeks. Results indicated that PCR is a useful tool that can be used in diagnosis of MD and RE in affected tissue stored as FFPE tissue blocks or in those only preserved in formalin. The advantages of using PCR in confirming diagnosis of MD in formalin preserved tissues will be discussed.

Last Modified: 9/21/2014
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