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United States Department of Agriculture

Agricultural Research Service

Research Project: Developing Resistance to Aflatoxin through Seed-Based Technologies

Location: Food and Feed Safety Research

Title: Biolistic transformation of cotton zygotic embryo meristem

Author
item Rajasekaran, Kanniah

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: August 14, 2012
Publication Date: January 7, 2013
Citation: Rajasekaran, K. 2013. Biolistic transformation of cotton zygotic embryo meristem. In: Zhang, B-H. (editor). Transgenic Cotton: Methods and Protocols, Methods in Molecular Biology. Humana Press. 958:47-57.

Technical Abstract: Biolistic transformation of cotton meristems, isolated from mature seed is detailed in this book chapter. This method is simple and avoids the necessity to use genotype-dependent regenerable cell cultures. However, identification of germ line transformation using this method is laborious and time-consuming. The detailed protocol on meristem transformation is described in this chapter for generating germ line transformation events as well as transformation of superficial layers (epidermis) of plant organs derived from bombarded cotton meristem. In addition, this procedure offers a quick method to evaluate gene constructs in cotton cells, organs, and tissues especially fibers which originate as single cells from the maternal epidermis layer of cotton ovules. This procedure will be useful to students, technicians, and scientists interested in cotton biotechnology.

Last Modified: 10/21/2014
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