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United States Department of Agriculture

Agricultural Research Service

Research Project: PLANT GENETIC RESOURCE AND INFORMATION MANAGEMENT

Location: North Central Regional Plant Introduction Station, Ames, Iowa

Title: Using High-Throughput Genotyping Information for Management of a Large Maize Collection

Authors
item Gardner, Candice
item Romay-Alvarez, Maria -
item Millard, Mark
item Flint-Garcia, Sherry
item Holland, Jim
item Buckler, Edward

Submitted to: Agronomy Society of America, Crop Science Society of America, Soil Science Society of America Meeting
Publication Type: Abstract Only
Publication Acceptance Date: June 28, 2012
Publication Date: October 21, 2012
Citation: Gardner, C.A., Romay-Alvarez, M.C., Millard, M.J., Flint Garcia, S.A., Holland, J.B., Buckler Iv, E.S. 2012. Using High-Throughput Genotyping Information for Management of a Large Maize Collection. Agronomy Society of America, Crop Science Society of America, Soil Science Society of America Meeting. Abstract 375-3.

Technical Abstract: The National Plant Germplasm System's collection of maize inbred lines is curated by the USDA-ARS North Central Regional Plant Introduction Station (NCRPIS) personnel in Ames, Iowa. The collection offers a rich set of genetic resources including key historical inbreds relevant to maize breeding history and breeding programs from all over the world. In 2010 or 2011, nearly 3000 lines were phenotypically characterized in Ames, IA, Columbia, MO, Ithaca, NY, and Raleigh, NC. These lines and additional lines from other sources were genotyped using Genotyping-By-Sequencing (GBS), a low cost, high-throughput sequencing technology. The method produced more than 1.3 million SNP markers distributed across the entire genome, with ability to call rare alleles at high confidence levels. Data analysis confirms high levels of genetic diversity among the lines, with extent of variation dependent on the different subpopulations, and clustering of very similar inbred lines. The power of this resource will be examined with respect to both the responsibilities and issues associated with managing a large collection of diverse genetic resources, and a researcher's ability to select germplasm with traits or alleles of interest.

Last Modified: 8/30/2014
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