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United States Department of Agriculture

Agricultural Research Service

Research Project: CHARACTERIZING, DETECTING, AND ELIMINATING PATHOGENS TO ENABLE THE SAFE INTRODUCTION OF PLANT GENETIC RESOURCES

Location: National Germplasm Resources

Title: Biological characterization and complete genomic sequence of Carrot thin leaf virus

Authors
item Xu, Donglin -
item Liu, Hsing Yeh
item Li, Fan -
item Tian, Tongyan -
item Li, Ruhui

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 4, 2013
Publication Date: November 6, 2013
Citation: Xu, D., Liu, H., Li, F., Tian, T., Li, R. 2013. Biological characterization and complete genomic sequence of Carrot thin leaf virus. Archives of Virology. European Journal of Plant Pathology 138:15-22.

Interpretive Summary: Several apiaceous species such as carrots and cilantro are important vegetable crops. Many viruses including Carrot thin leaf virus (CTLV) infect these crops and cause production problems. CTLV has been reported in Washington State and California in the United States. Although the virus has been known to cause disease for many years, its full genetic sequence had never been determined. In this study, the host range and reactions, aphid transmission of a cilantro isolate of CTLV from California was determined. CTLV is very similar to several other viruses in host ranges, but it induces distinct thin leaf symptom on many of its hosts. Analysis of the genetic information shows that CTLV is distinct from other related viruses. This study provides information necessary for the proper grouping and naming of the virus. It also assists with the development of better diagnostic and management tools for these viruses and the diseases they cause in vegetable production for vegetable farmers.

Technical Abstract: The host range of a cilantro isolate of Carrot thin leaf virus (CTLV-Cs) was determined to include 15 plant species. The virus was also transmitted to 9 of 11 tested apiaceous species by aphids. Complete genomic sequences of CTLV-Cs and a carrot isolate of CTLV were determined. Their genomic sequences are 98.1% identical. The CTLV genomic organization is typical of potyviruses, and contains conserved motifs found in the genus Potyvirus. Pairwise comparisons show that the virus shares 52.0-59.6% identities with those of other members in the genus Potyvirus at genomic sequence level. Phylogenetic analyses confirm that CTLV is a distinct potyvirus.

Last Modified: 11/25/2014
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